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Enzyme evolution in a microbial community growing on the herbicide Dalapon

Abstract

A seven-membered microbial community capable of utilising the herbicide Dalapon has been isolated by continuous-flow enrichment culture. The composition of this community has remained remarkably stable over thousands of hours in a Dalapon-limited chemostat. During this period, however, one member of the community, Pseudomonas putida, acquired the ability to grow on Dalapon through the evolution of an extant dehalogenase.

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References

  1. Veldkamp, H., and Jannasch, H. W., J. appl. Chem. Biotechnol., 22, 105–123 (1972).

    Article  CAS  Google Scholar 

  2. Veldkamp, H., Sixth International Symposium on Continuous Culture (edit. by Dean, A. C. R., Ellwood, D. C., Evans, C. G. T., and Melling, J.) (in the press, 1976).

    Google Scholar 

  3. Kearney, P. C., Kaufman, D. D., and Beall, M. L., Biochem. biophys. Res. Commun., 14, 29–33 (1964).

    Article  CAS  Google Scholar 

  4. Shewan, J., Hobbs, G., and Hodgkiss, W., J. appl. Bact., 23, 379–390 (1960).

    Article  Google Scholar 

  5. Powell, E. O., J. gen. Microbiol., 18, 259–268 (1958).

    Article  CAS  Google Scholar 

  6. Slater, J. H., Bull, A. T., and Senior, E., Proc. Soc. gen. Microbiol., 3, 113 (1976).

    Google Scholar 

  7. Clarke, P. H., in Evolution in the Microbial World (edit. by Carlile, M. J., and Skehel, J. J.), (Cambridge University Press, London, 1974).

    Google Scholar 

  8. Hartley, B. S., in Evolution in the Microbial World (edit. by Carlile, M. J., and Skehel, J. J.), (Cambridge University Press, London, 1974).

    Google Scholar 

  9. Vogel, A. I., A Textbook of Quantitative Inorganic Analysis, third ed. (Longman, London, 1962).

    Google Scholar 

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Senior, E., Bull, A. & Slater, J. Enzyme evolution in a microbial community growing on the herbicide Dalapon. Nature 263, 476–479 (1976). https://doi.org/10.1038/263476a0

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  • DOI: https://doi.org/10.1038/263476a0

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