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Specific cleavage of chloroplast DNA from higher plants by EcoRI restriction nuclease

Nature volume 263, pages 440–442 (1976)Cite this article

Abstract

THE physicochemical properties of chloroplast (cp)–DNA in higher plants have been reported1–4. Cp–DNAs occur as closed circles of molecular weight 85×106–97×106. Specific identification of cp–DNAs isolated from different plants cannot, however, be carried out on the basis of buoyant density, melting points, kinetic complexity or direct length measurement by electron microscopy, since each of these techniques give values which fall within narrow limits, and only overlap with one another a little1,2.

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire Végétale, associé an CNRS (L.A. 40) Bât. 430, Université Paris-Sud, 91405, ORSAY, France

    FERNAND VEDEL, FRANCIS QUETIER & MARCEL BAYEN

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  1. FERNAND VEDEL
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  2. FRANCIS QUETIER
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  3. MARCEL BAYEN
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VEDEL, F., QUETIER, F. & BAYEN, M. Specific cleavage of chloroplast DNA from higher plants by EcoRI restriction nuclease. Nature 263, 440–442 (1976). https://doi.org/10.1038/263440a0

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