Abstract
WHILE attempting to isolate hydra morphogens by column chromatography of tissue homogenates, I decided that a bacteria-free culture would be advantageous. Having established that a final concentration of 100 µg ml−1 penicillin G plus 100 µg ml−1 streptomycin in the normal1 culture medium M gave a 100-fold reduction in bacterial contamination in 24 h, I found that hydra could not feed in this solution; indeed, they seemed unaware of Artemia larvae bumping into them. On transferring the hydra to fresh medium without antibiotic they were immediately able to feed normally.
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WILBY, O. Nematocyst discharge and the effects of antibodies on feeding in hydra. Nature 262, 387–388 (1976). https://doi.org/10.1038/262387a0
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DOI: https://doi.org/10.1038/262387a0
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