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In vivo evidence for a circadian rhythm in membranes of Gonyaulax

Nature volume 261, pages 583–585 (1976)Cite this article

Abstract

IT has been suggested that rhythmic oscillations in membranes are the underlying mechanism for endogenous circadian rhythms1,2 To support this suggestion, periodic oscillations in membranes of organisms displaying circadian rhythmicity must first be demonstrated. Rhythmic oscillations associated with membranes have been observed in the spontaneous firing of the optic nerve of Aplysia3 and in the transmembrane potential of the pulvini of Samanea4. It is not clear if these membrane-associated rhythms are a function of specialised cells, or are basic phenomena of circadian rhythms. The only membrane-associated rhythm described in a unicellular organism so far is that of a circadian rhythm in particle distribution of one of the membranes of Gonyaulax polyedra5. We have now investigated the rhythmic changes in the membrane potential of G. polyedra. We used the method of Hoffman and Laris6, in which fluorescent cyanine dyes are used to monitor the membrane potential in vivo of cells not amenable to the insertion of microelectrodes, in this case because of their small size and lack of a central vacuole. Our results suggest a temporal reorganisation of one of the membranes of Gonyaulax with circadian time.

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References

  1. Sweeney, B. M., Int. J. Chronobiol., 2, 25–33 (1974).

    CAS  PubMed  Google Scholar 

  2. Njus, D., Sulzman, F. M., and Hastings, J. W., Nature, 248, 116–120 (1974).

    Article  ADS  CAS  Google Scholar 

  3. Jacklet, J. W., Science, 164, 562–563 (1969).

    Article  ADS  CAS  Google Scholar 

  4. Racusen, R., and Satter, R. L., Nature, 255, 408–410 (1975).

    Article  ADS  CAS  Google Scholar 

  5. Sweeney, B. M., Am. Inst. biol. Sci. meeting, 1975 (Abstr.).

  6. Hoffman, J. F., and Laris, P. G., J. Physiol., Lond., 239, 519–552 (1974).

    Article  CAS  Google Scholar 

  7. Guillard, R. R. L., and Ryther, J. H., Can. J. Microbiol., 8, 229–239 (1962).

    Article  CAS  Google Scholar 

  8. Laris, P. C., Bahr, D. P., and Jaffee, R. R. J., Biochim. biophys. Acta, 376, 415–475 (1975).

    Article  CAS  Google Scholar 

  9. Löeblich, A. R., III, Proc. N. Am. Paleontol. Convention, Part G, 867–929 (1969).

  10. Adamich, M., and Sweeney, B. M., Planta (in the press).

  11. Gaff, D. F., and OKong'O-Ogola, O., J. exp. Bot., 22, 756–758 (1971).

    Article  Google Scholar 

  12. Stadleman, E. J., and Kinzel, H., in Methods in Cell Physiology (edit. by Prescott, D. M.), 325–372 (Academic, New York, 1972).

    Google Scholar 

  13. Laris, P. C., Pershadsingh, H. A., and Johnstone, R. M., J. gen. Physiol., 66, 14a (1975).

    Google Scholar 

  14. Sims, P. J., Waggoner, A. S., Wang, C. H., and Hoffman, J. F., Biochemistry, 13, 3315–3330 (1974).

    Article  CAS  Google Scholar 

  15. Huebner, J. S., Biochim. biophys. Acta, 406, 178–186 (1975).

    Article  CAS  Google Scholar 

  16. Sweeney, B. M., Pl. Physiol., Lancaster, 53, 337–342 (1974).

    Article  CAS  Google Scholar 

  17. Lowry, O. H., Rosebrough, N. J., Farr, L. A., and Randall, R. J., J. biol. Chem., 193, 265–275 (1951).

    CAS  Google Scholar 

  18. Undenfriend, S., Stein, S., Böhlen, P., and Dairman, W., Science, 178, 871–872 (1972).

    Article  ADS  Google Scholar 

  19. DeGier, J., Haest, C. W. M., Mandersloot, G., and Van Deenen, L. L. M., Biochem. biophys. Acta, 211, 373–375 (1970).

    Article  CAS  Google Scholar 

  20. Van Deenen, L. L. M., Fedn Proc., 30, 1032 (1971).

    Google Scholar 

  21. Scarpa, A., and DeGier, J., Biochem. biophys. Acta, 241, 789–797 (1971).

    Article  CAS  Google Scholar 

  22. DeGier, J., and Scarpa, A., Abstr. FEBS Meet., 520 (1971).

  23. Korasne, S., Eisenman, G., and Szabo, G., Science, 174, 412–415 (1971).

    Article  ADS  Google Scholar 

  24. Stark, G., Benz, R., Pohl, G. W., and Janko, K., Biochim. biophys. Acta, 266, 603–612 (1972).

    Article  CAS  Google Scholar 

  25. Adamich, M., Sweeney, B. M., ICN-UCLA Winter Conf. molec. Cell Biol., No. 68, 42 (University of California, Los Angeles, 1976).

  26. Stadelman, E. J., A. Rev. Pl. Physiol., 20, 585–606 (1969).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biological Sciences, University of California, Santa Barbara, Santa Barbara, California, 93106

    MARINA ADAMICH, PHILIP C. LARIS & BEATRICE M. SWEENEY

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  1. MARINA ADAMICH
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  2. PHILIP C. LARIS
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  3. BEATRICE M. SWEENEY
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ADAMICH, M., LARIS, P. & SWEENEY, B. In vivo evidence for a circadian rhythm in membranes of Gonyaulax. Nature 261, 583–585 (1976). https://doi.org/10.1038/261583a0

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