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Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter

Abstract

NITROGEN fixation mutants have been isolated and biochemically characterised in Azotobacter vinelandii1, but the absence of chromosomal gene transfer systems such as conjugation in this genus has prevented a genetic analysis of such mutants. We now report the intergeneric transfer of Klebsiella nif genes carried on the P-type plasmid, RP41 (refs 2 and 3), to Azotobacter, and their expression in this host.

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CANNON, F., POSTGATE, J. Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter. Nature 260, 271–272 (1976). https://doi.org/10.1038/260271a0

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  • DOI: https://doi.org/10.1038/260271a0

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