Abstract
OOCYTE injection as developed by Gurdon et al.1–3 is efficient and reliable for the assay of faithful translation of heterologous messenger RNA2–6. But, working in collaboration with Gurdon7, we found that oncogenic viral RNA was not translated to a detectable extent in oocytes. Only in a cell-free system derived from Escherichia coli could we translate viral RNA into distinct polypeptides in the molecular weight range of the viral group-specific proteins8. Recently we achieved translation of Rauscher leukaemia virus (RLV) RNA and avian myeloblastosis virus (AMY) RNA in various cell-free systems, using immunoprecipitation for the analysis9,10. This verified that our failure to find virus-specific polypeptides in the oocyte system programmed with oncogenic viral RNA was due to the inadequacy of the methods available to detect translation when it occurs at low efficiency. We now report the synthesis of AMV-specific precursors and structural proteins in the oocyte system, detected by specific immunoprecipitation and scintillation autoradiography.
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SALDEN, M., ASSELBERGS, F. & BLOEMENDAL, H. Translation of oncogenic virus RNA in Xenopus laevis oocytes. Nature 259, 696–699 (1976). https://doi.org/10.1038/259696a0
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DOI: https://doi.org/10.1038/259696a0
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