Abstract
WITH recent advances in nucleic acid biochemistry, it has become possible to recombine different animal viral DNAs at specific sites in vitro1,2. Specific hybrid viral genomes can be used in the genetic analysis of animal tumour viruses. With this aim in mind, we have made hybrids of two strains of polyoma (A-3 and P16). A-3 and P16 were developed from independent isolates and have been characterised both phenotypically and genotypically3,4. Using the hybrids it has been possible to begin to locate the DNA regions coding for phenotypic differences in the parent strains.
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MILLER, L., FRIED, M. Construction of infectious polyoma hybrid genomes in vitro. Nature 259, 598–601 (1976). https://doi.org/10.1038/259598a0
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DOI: https://doi.org/10.1038/259598a0
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