Abstract
WE postulate that eukaryotic DNA replication occurs at discrete intranuclear macrostructures, replication complexes, which might be separable from the bulk of nuclear material. A complex composed of DNA, protein and other nuclear constituents could have a different intrinsic density from nucleic acids or protein alone, and this might be the basis for its isolation. Replication complexes would characteristically contain nascent DNA1–4 and DNA polymerase. If they are functionally intact, they should also have the intrinsic capacity to synthesise DNA. We have used a previously reported method for the fractionation of sonically disrupted nuclei on gradients of Cs2SO4 (ref. 5). The results indicate that replication complexes may be partially purified by this method, and that ATP-dependent DNA synthesis is a characteristic of the complexes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Painter, R. B., and Schaefer, A., Nature, 221, 1215–1217 (1969).
Habener, J. F., Bynum, B. S., and Shack, J., J. molec. Biol., 49, 157–170 (1970).
Sato, S., Ariake, S., Saito, M., and Sugimura, T., Biochem. biophys. Res. Commun., 49, 270–277 (1972).
Probst, H., and Jenke, H.-S., Biochem. biophys. Res. Commun., 52, 800–806 (1973).
Genta, V. M., Kaufman, D. G., and Kaufmann, W. K., Analyt. biochem., 67.
Grisham, J. W., Cancer Res., 22, 842–849 (1962).
Bernardi, G., Nature, 206, 779–783 (1965).
Dingman, C. W., Fisher, M. P., and Kakefuda, T., Biochemistry, 11, 1242–1250 (1972).
Dingman, C. W., Fisher, M. P., and Ishizawa, M., J. molec. Biol., 84, 275–295 (1974).
Lynch, W. E., Brown, R. F., Umeda, T., Langreth, S. G., and Lieberman, I., J. biol. Chem., 245, 3911–3916 (1970).
Grisham, J. W., Kaufman, D. G., and Stenstrom, M. L., Biochem. biophys. Res. Commun., 49, 420–427 (1972).
Kaufman, D. G., Grishman, J. W., and Stenstrom, M. L., Biochim. biophys. Acta, 272, 212–219 (1972).
Hershey, H. V., Stieber, J. F., and Mueller, G. C., Eur. J. Biochem., 34, 383–394 (1973).
Infante, A. A., Nauta, R., Gilbert, S., Hobart, P., and Firshein, W., Nature new Biol., 242, 5–8 (1973).
Hanaoka, F., and Yamada, M., Biochem. biophys. Res. Commun., 42, 647–653 (1973).
Hildebrand, C. E., and Tobey, R. A., Biochim. biophys. Acta, 331, 165–180 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GENTA, V., KAUFMAN, D., KAUFMANN, W. et al. Eukaryotic DNA replication complex. Nature 259, 502–503 (1976). https://doi.org/10.1038/259502a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/259502a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.