Abstract
ONLY one rat liver ribosomal protein (S6) is phosphorylated in vivo1. During hepatic regeneration the phosphorylation of S6 is increased by an order of magnitude, and derivatives are generated (in some experiments as many as five) which contain increasing numbers of phosphoserine residues1. Very similar changes in the phosphorylation of S6 are caused by administration to animals of glucagon or cyclic AMP (A.M.G. and I.G.W., unpublished). Indeed, a number of hormones including adrenocorticotrophic hormone (ACTH)2 and thyroid hormone3 increase the phosphorylation of ribosomal protein, although in the latter instances, the identity of the phosphoprotein has not been established. These observations have led us to consider the possibility that cyclic AMP is the mediator of the phosphorylation of S6 (ref. 4). The concentration of cyclic AMP in the liver is increased in diabetic animals and restored to normal by insulin5, although it is still moot whether the effect of the hormone on the level of the cyclic nucleotide accounts for all (or even any) of its actions on hepatic cells6. We have tested the effect of experimental diabetes and of insulin administration on the phosphorylation of rat liver ribosomal protein: the phosphorylation of S6 was markedly increased by diabetes, and reduced towards the normal level by insulin administration.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gressner, A. M., and Wool, I. G., J. biol. Chem., 249, 6917–6925 (1974).
Roos, B. A., Endocrinology, 93, 1287–1293 (1973).
Correze, C., Pinell, P., and Nunez, J., FEBS Lett., 23, 87–91 (1972).
Gressner, A. M., and Wool, I. G., Biochem. biophys. Res. Commun., 60, 1482–1490 (1974).
Jefferson, L. S., Exton, J. H., Butcher, R. W., Sutherland, E. W., and Park, C. R., J. biol. Chem., 243, 1031–1038 (1968).
Park, C. R., Lewis, S. B., and Exton, J. H., Diabetes, 21, (suppl. 2) 439–446 (1972).
Martin, T. E., and Wool, I. G., J. molec. Biol., 43, 151–161 (1969).
Stirewalt, W. S., Castles, J. J., and Wool, I. G., Biochemistry, 10, 1594–1598 (1971).
Sherton, C. C., and Wool, I. G., Molec. gen. Genet., 135, 97–112 (1974).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265–275 (1951).
Kaltschmidt, E., and Wittmann, H. G., Analyt. Biochem., 36, 401–412 (1970).
Exton, J. H., Harper, S. C., Tucker, A. L., and Ho, R.-J., Biochim. biophys. Acta, 329, 23–40 (1973).
Sherton, C. C., and Wool, I. G., J. biol. Chem., 247, 4460–4467 (1972).
Blat, C., and Loeb, J. E., FEBS Lett., 18, 124–126 (1971).
Majumder, G. C., and Turkington, R. W., J. biol. Chem., 247, 7207–7217 (1972).
Tragl, K. H., and Reaven, G. M., Diabetes, 20, 27–32 (1971).
Peterson, D. T., Alford, F. P., Reaven, E. P., Veyama, I., and Reaven, G. M., J. clin. Invest., 53, 3201–3211 (1973).
Pilkis, S. J., and Korner, A., Biochim. biophys. Acta, 247, 597–608 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GRESSNER, A., WOOL, I. Effect of experimental diabetes and insulin on phosphorylation of rat liver ribosomal protein S6. Nature 259, 148–150 (1976). https://doi.org/10.1038/259148a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/259148a0
This article is cited by
-
The MAP kinase cascade. Discovery of a new signal transduction pathway
Molecular and Cellular Biochemistry (1993)
-
Phosphorylation of ribosomal protein S6 in suspension cultured HeLa cells
Molecular and General Genetics MGG (1977)
-
Factors involved in initiation of haemoglobin synthesis can be phosphorylated in vitro
Nature (1976)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.