Abstract
TECHNIQUES have been developed for the growth of haemopoietic cells (see refs 1 and 2), human T lymphocytes3 and mouse B lymphocytes4 on soft agar. Experiments with mouse lymphocytes suggest that concanavalin A (con A) and phytohaemagglutinin (PHA) stimulate T cells5,6, whereas pokeweed induces proliferation of both B and T cells7. Based on previously described techniques for obtaining clonal growth of human lymphocyte colonies in vitro3, a procedure has now been developed for inducing clonal proliferation of mitogen-stimulated mouse lymphocytes using a two-layer soft agar technique.
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References
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SREDNI, B., KALECHMAN, Y., MICHLIN, H. et al. Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes. Nature 259, 130–132 (1976). https://doi.org/10.1038/259130a0
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DOI: https://doi.org/10.1038/259130a0
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