Letter | Published:

Incorporation of GMP into specific tRNA molecules by extracts of Ehrlich ascites tumour cells

Nature volume 257, pages 327329 (25 September 1975) | Download Citation

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Abstract

ENZYMATIC activities for synthesising four ribohomopolymers, including poly(G), have been found in rat liver, and all are stimulated by RNA1,2. Recently two poly(G) polymerases were isolated from plant cells; one requires poly(G) as a primer3 and the other denatured DNA4. We report here the isolation of an enzyme from Ehrlich ascites tumour, which catalyses the incorporation of GMP from GTP into an acid-insoluble product. This enzyme requires specific tRNAs as primers. Our data suggest that the tRNAs which can serve as primers are specific to tumours or tissues infected with tumour viruses.

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Affiliations

  1. Department of Molecular Biology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan

    • TATEO ITOH
    • , ICHIRO HARUNA
    •  & ITARU WATANABE

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DOI

https://doi.org/10.1038/257327a0

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