Letter | Published:

Induction of plants from pollen grains of Petunia cultured in vitro

Naturevolume 257pages222224 (1975) | Download Citation



THE ease with which plantlets can be produced from somatic tissue, protoplasts and anther cultures of Petunia1–3 has prompted us to use isolated pollen grain culture4 to determine precisely the stage at which pollen elicits the best androgenic response, with a view to increasing the yield of haploid plants thus produced.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Rao, P. S., Handro, W., and Harada, H., Physiol. Pl., 28, 458–463 (1973).

  2. 2

    Durand, J., Potrikus, I., and Donn, G., Z. Pflanzenphysiol., 69, 26–34 (1973).

  3. 3

    Raquin, C., and Pilet, V., C. r. hebd. Seanc. Acad. Sci. Paris, 274, 1019–1022 (1972).

  4. 4

    Nitsch, C., and Norreel, B., in Genes, Enzymes and Population, 129–145 (Plenum Press, 1972).

  5. 5

    Murashige, T., and Skoog, F., Physiol. Pl., 15, 473–497 (1962).

  6. 6

    Nitsch, J. P., and Nitsch, C., Ann. Physiol. vég., 7, 251–256 (1965).

  7. 7

    Melchers, G., and Labib, G., Molec. gen. Genet., 135, 272–274 (1974).

  8. 8

    Narayanaswamy, S., and Chandy, L. P., Ann. Bot., 35, 535–542 (1971).

  9. 9

    Wagner, G., and Hess, D., Z. Pfl. Physiol., 73, 273–276 (1974).

  10. 10

    Sunderland, N., and Dunwell, J. M., in Tissue Culture and Plant Science (edit. by Street, H. E.), 141–167 (Academic, London, 1974).

Download references

Author information

Author notes

    • B. NORREEL

    Present address: Laboratoire de Cytologie et Morphogénèse végétales, Université Pierre et Marie Curie, Bât. L, 9 quai Saint Bernard, 75005, Paris, France


  1. Laboratoire de Physiologie Pluricellulaire CNRS, 91190, Gif-sur-Yvette, France

    • R. S. SANGWAN
    •  & B. NORREEL


  1. Search for R. S. SANGWAN in:

  2. Search for B. NORREEL in:

About this article

Publication history



Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.