Abstract
Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+andsu3−)and tRNA2Tyr, tRNA2Gly(su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Altman, S., Nature new Biol., 229, 19–21 (1971).
Schedl, P., and Primakoff, P., Proc. natn. Acad. Sci. U.S.A., 70, 2091–2095 (1973).
Sakano, H., Yamada, S., Ikemura, T., Shimura, Y., and Ozeki, H., Nucl. Acid Res., 1, 355–371 (1974).
Burdon, R. H., Prog. Nucl. Acid Res. molec. Biol., 11, 33–73 (Academic, New York, 1971).
McClain, W. H., Guthrie, C., and Barrell, B. G., Proc. natn. Acad. Sci. U.S.A., 69 3703–3707 (1972).
Guthrie, C., et al., Nature new Biol., 246, 6–11 (1973).
Altman, S., and Smith, J. D., Nature new Biol., 233, 35–39 (1971).
Carbon, S., et al., Brookhaven Symp. Biol. (in the press).
Barrell, B. G., Seidman, J. G., Guthrie, C., and McClain, W. H., Proc. natn. Acad. Sci. U.S.A., 71, 413–416 (1974).
Russell, R. L., et al., J. molec. Biol., 47 1–13 (1970).
Squires, C., and Carbon, J., Nature new Biol., 233 274–277 (1971).
Squires, C., Konrad, B., Kirschbaum, J., and Carbon, J., Proc. natn. Acad. Sci. U.S.A., 70, 438–441 (1973).
Andoh, T., and Ozeki, H., Proc. natn. Acad. Sci. U.S.A., 59 792–799 (1968).
Ghysen, A., and Celis, J. E., Nature, 249, 418–421 (1974).
Daniel, V., Sarid, S., Beckmann, J. S., and Littauer, U. Z., Proc. natn. Acad. Sci. U.S.A., 66, 1260–1266 (1970).
Grimberg, J. I., and Daniel, V., Nature, 250, 320–323 (1974).
Peacock, A. C., and Dingman, C. W., Biochemistry, 7, 668–674 (1968).
Chamberlin, M., and Berg, P., Proc. natn. Acad. Sci. U.S.A., 48, 81–94 (1962).
Burgess, R. R., J. biol. Chem., 244, 6160–6167 (1969).
Roberts, J. W., Nature, 244, 1168–1174 (1969).
Sanger, F., Brownlee, G., and Barrell, B., J. molec. Biol., 13, 373–398 (1965).
Goodman, H. M., Abelson, J., Landy, A., Brenner, S., and Smith, J. D., Nature, 217, 1019–1024 (1968).
Roberts, J. W., and Carbon, J., Nature, 250, 412–414 (1974).
Clarke, L., and Carbon, J., J. biol. Chem., 249, 6874–6885 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Daniel, V., Grimberg, J. & Zeevi, M. In vitro synthesis of tRNA precursors and their conversion to mature size tRNA. Nature 257, 193–197 (1975). https://doi.org/10.1038/257193a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/257193a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.