Letter | Published:

Aminoacylation of transfer RNA microinjected into Xenopus laevis oocytes

Naturevolume 256pages675678 (1975) | Download Citation

Subjects

Abstract

THE microinjection technique perfected by Gurdon and his collaborators1,2 represents an excellent method for the study of the in vivo effects of drastically changing the molecular contents of the injected cells. We have demonstrated3 that radioactive yeast transfer RNA injected into oocytes of Xenopus laevis is not appreciably degraded after 20 h of incubation inside these cells. It seems therefore, possible to modify the quantity and characteristics of the tRNAs present in frog oocytes and to test the effects that such changes may have on protein synthesis and other cellular processes. An important control in such a study is to determine whether the tRNAs microinjected into frog oocytes are functional in the sense that they can be aminoacylated by the recipient cell. This report presents evidence demonstrating that the amphibian oocyte is capable of aminoacylating yeast tRNAs injected into these cells at concentrations which are greatly in excess of their endogenous tRNA content.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gurdon, J. B., J. Embryol. exp. Morph., 20, 401–414 (1968).

  2. 2

    Gurdon, J. B., Lane, C. D., Woodland, H. R., and Marbaix, G., Nature, 233, 177–182 (1971).

  3. 3

    Allende, C. C., Allende, J. E., and Firtel, R., Cell, 2, 189–196 (1974).

  4. 4

    Tarragó, A., Allende, J. E., Redfield, B., and Weissbach, H., Archs Biochem. Biophys., 159, 353–361 (1973).

  5. 5

    Peacock, A. C., and Dingman, C. W., Biochemistry, 7, 668–674 (1968).

  6. 6

    Jerez, C., Sandoval, A., Allende, J. E., Henes, C., and Ofengard, J., Biochemistry, 8, 3006–3014 (1969).

  7. 7

    Legocki, A., and Marcus, A., J. biol. Chem., 245, 2814–2818 (1970).

  8. 8

    Brown, D. D., and Littna, E., J. molec. Biol., 20, 95–112 (1966).

  9. 9

    Airhart, J., Vidrich, A., and Khairallah, E. A., Biochem. J., 140, 539–548 (1974).

  10. 10

    Van Venrooij, W. J., Moonen, H., and Van Loon-Klaasen, L., Eur. J. Biochem., 297–304 (1974).

  11. 11

    Baldwin, A. N., and Berg, P., J. biol. Chem., 241, 839–845 (1966).

  12. 12

    Brungraber, E. E., Biochem. biophys. Res. Commun., 8, 1–3 (1962).

  13. 13

    Tarrago, A., Monasterio, O., and Allende, J. E., Biochem. biophys. Res. Commun., 41, 765–773 (1970).

  14. 14

    Muench, K., and Berg., P., in Procedures in Nucleic Acid Research (edit. by Cantoni, G. L., and Davies, D. R.), 375–383 (Harper and Row, New York, 1966).

Download references

Author information

Affiliations

  1. Departamento de Bioquimica, Facultad de Medicina, Sede Norte Universidad de Chile, Casilla, 6671, Santiago, 4, Chile

    • MARTA GATICA
    • , ADELA TARRAGÓ
    • , CATHERINE C. ALLENDE
    •  & JORGE E. ALLENDE

Authors

  1. Search for MARTA GATICA in:

  2. Search for ADELA TARRAGÓ in:

  3. Search for CATHERINE C. ALLENDE in:

  4. Search for JORGE E. ALLENDE in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/256675a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.