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Stimulation by cyclic GMP of lymphocytes mediated by soluble factor released from adherent cells

Naturevolume 256pages418419 (1975) | Download Citation

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Abstract

THE activation of lymphocytes by mitogens is a complex phenomenon which can be subdivided into reactions occurring on the surface of the cells1 followed by a chain of intracellular reactions caused by biochemical alterations in the membrane2. With respect to the mediation of the mitogenic signal from the membrane to the nucleus it has been claimed that 3′,5′ – cyclic GMP either directly fulfils a role as a ‘second messenger’3, or that a 3′,5′–cyclic GMP-dependent step is involved in the chain of intracellular reactions culminating in cell mitosis3,4. These assumptions are based on the facts that after the addition of lectins to peripheral white blood cells depleted of granulocytes and monocytes, an increased intracellular concentration of 3′,5′–cyclic GMP was detected3 and that 3′,5′–cyclic GMP added exogenously activated DNA synthesis in spleen and thymus cells in vitro4–6. The prerequisites for the validity of the above mentioned hypothesis, however, are that the 3′,5′–cyclic GMP levels are indeed increased in all cells entering the mitotic phase and that 3′,5′–cyclic GMP added exogenously acts directly on the lymphocytes, without the participation of other cells.

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Affiliations

  1. Immunological Research Unit, Klinikum Steglitz, Freie Universität, Hindenburgdamm 30, D-1, Berlin, 45, Germany

    • TIBOR DIAMANTSTEIN
    •  & ARTUR ULMER

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https://doi.org/10.1038/256418a0

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