Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli

Nature volume 256, pages 183–187 (1975)Cite this article

Abstract

Transcription of ribosomal protein genes in the str-spc region in Escherichia coli seems to be exclusively anticlockwise. Insertion mutations in this region inactivate many but not all distal ribosomal protein genes. Thus, there seems to be more than one transcriptional unit for the ribosomal protein genes in this region.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Jaskunas, S. R., Nomura, M., and Davies, J., in Ribosomes (edit. by Nomura, M., Tissières, A., and Lengyel, P.), 333–368 (Cold Spring Harbor Laboratory, New York, 1974).

    Google Scholar 

  2. Jaskunas, S. R., Lindahl, L., and Nomura, M., Proc. natn. Acad. Sci. U.S.A., 72, 6–10 (1975).

    Article  ADS  CAS  Google Scholar 

  3. Nomura, M., and Engbaek, F., Proc. natn. Acad. Sci. U.S.A., 69, 1526–1530 (1972).

    Article  ADS  CAS  Google Scholar 

  4. Hirsch, H. J., Starlinger, P., and Brachet, P., Molec. gen. Genet., 119, 191–206 (1972).

    Article  CAS  Google Scholar 

  5. Malamy, M. H., Fiandt, M., and Szybalski, W., Molec. gen. Genet., 119, 207–222 (1972).

    Article  CAS  Google Scholar 

  6. Saedler, H., Reif, H. J., Hu, S., and Davidson, N., Molec. gen. Genet., 132, 265–289 (1974).

    Article  CAS  Google Scholar 

  7. Molin, S., von Meyenburg, K., Gulløv, K., and Maaløe, O., Molec. gen. Genet., 129, 11–26 (1974).

    Article  CAS  Google Scholar 

  8. Bollen, A., et al., J. molec. Biol., 76, 463–472 (1973).

    Article  CAS  Google Scholar 

  9. Kahan, L., Zengel, J., Nomura, M., Bollen, A., and Herzog, A., J. molec. Biol., 76, 473–483 (1973).

    Article  CAS  Google Scholar 

  10. Dennis, P., J. molec. Biol., 88, 25–41 (1974).

    Article  CAS  Google Scholar 

  11. Dennis, P., and Nomura, M., Proc. natn. Acad. Sci. U.S.A., 71, 3819–3823 (1974).

    Article  ADS  CAS  Google Scholar 

  12. Murialdo, H., and Siminovitch, L., in The Bacteriophage Lambda (edit. by Hershey, A. D.), 711–723 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  13. Kaltschmidt, E., and Wittmann, H. G., Analyt. Biochem., 36, 401–412 (1970).

    Article  CAS  Google Scholar 

  14. Kaltschmidt, E., Kahan, L., and Nomura, M., Proc. natn. Acad. Sci. U.S.A., 71, 446–450 (1974).

    Article  ADS  CAS  Google Scholar 

  15. Bøvre, K., Lozeron, H. A., and Szybalski, W., in Methods in Virology (edit. by Maramorosch, K., and Koprowski, H.), 5, 271–292 (Academic, New York, 1971).

    Google Scholar 

  16. Davis, R. W., Simon, M., and Davidson, N., Methods in Enzymology (edit. by Grossman, L., and Moldave, K.), XXI, 413–428 (Academic, New York, 1971).

    Google Scholar 

  17. Reiner, A. M., J. Bact., 97, 1437–1443 (1969).

    PubMed  CAS  Google Scholar 

  18. Avitabile, A., et al., Molec. gen. Genet., 132, 1–12 (1974).

    Article  CAS  Google Scholar 

  19. Espejo, R. T., Canelo, E. S., and Sinsheimer, R. L., Proc. natn. Acad. Sci. U.S.A., 63, 1164–1168 (1969).

    Article  ADS  CAS  Google Scholar 

  20. Sharp, P. A., Hsu, M. T., Ohtsubo, E., and Davidson, N., J. molec. Biol., 71, 471–497 (1972).

    Article  CAS  Google Scholar 

  21. Sparling, P. F., Modolell, J., Takeda, Y., and Davis, B. D., J. molec. Biol., 37, 407–421 (1968).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Enzyme Research, Departments of Genetics and Biochemistry, University of Wisconsin, Wisconsin, 53706

    S. R. Jaskunas, L. Lindahl & M. Nomura

Authors
  1. S. R. Jaskunas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Lindahl
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Nomura
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jaskunas, S., Lindahl, L. & Nomura, M. Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli. Nature 256, 183–187 (1975). https://doi.org/10.1038/256183a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/256183a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing