Abstract
THE major histocompatibility complex of the mouse, known as the H-2 locus, represents the prime barrier against successful tissue transplants1 and controls the expression of many cell surface alloantigens which are serologically detectable2,3. It may be subdivided by recombinational analysis into four major regions known as K, D, Ir and Ss (ref. 4). The Ss region controls the quantitative expression of a serum protein5. The Ir region controls immune responsiveness of mice to a variety of synthetic and natural antigens6,7, and the expression of multiple cell surface proteins, some of which may be distinguished by molecular weight8,9. The K and D regions both control the expression of distinct cell surface glycoproteins (molecular weight 47,000), which are non-covalently associated with a small molecular weight (11,500) component10–11. Because the native molecules are hydrophobic proteins present on the cell surfaces in small quantities, it is necessary to use unusual procedures for their isolation and chemical characterisation. We have, therefore, used indirect immunoprecipitation and sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE) for the isolation and subsequent amino acid sequence analysis of both molecular weight components.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Counce, S., Smith, P., Barth, R., and Snell, G. D., Ann. Surg., 144, 198–204 (1956).
Amos, D. B., Gorer, P. A., and Mikelska, Z. B., Proc. R. Soc., B 144, 369–380 (1955).
David, C. S., Shreffler, D. C., and Frelinger, J. A., Proc. natn. Acad. Sci. U.S.A., 70, 2509–2514 (1973).
Klein, J., and Shreffler, D. C., Transplant. Rev., 6, 3–29 (1971).
Shreffler, D. C., and Passmore, H. C., in Proc. Symp. Immunogenet. H-2 Sys., 58–68 (S. Karger AG, Basel).
McDevitt, H. O., et al., J. exp. Med., 135, 1259–1278 (1972).
Lieberman, R., and Humphrey, W. J., J. exp. Med., 136, 1222–1230 (1972).
Cullen, S. E., David, C. S., Shreffler, D. C., and Nathenson, S. G., Proc. natn. Acad. Sci. U.S.A., 71, 648–652 (1974).
Silver, J., Sibley, C., Morand, P., and Hood, L., Transplant. Proc. (in the press).
Schwartz, B. D., Kato, K., Cullen, S. E., and Nathenson, S. G., Biochemistry, 12, 2157–2164 (1973).
Silver, J., and Hood, L., Nature, 249, 764–765 (1974).
Hermodson, M. A., Dricsson, L. H., Titani, K., Neurath, H., and Walsh, K. A., Biochemistry, 11, 4493–4501 (1972).
Grey, H. M., et al., J. exp. Med., 138, 1608–1612 (1973).
Cunningham, B. A., Wang, J. L., Berggard, I., and Peterson, P. A., Biochemistry, 12, 4811–4822 (1973).
Cunningham, B. A., and Berggard, I., Science, 187, 1079–1080 (1975).
Smithies, O., and Poulik, M. D., Proc. natn. Acad. Sci. U.S.A., 69, 2914–2917 (1972).
Poulik, M. D., Shinnick, E. S., and Smithies, O., Fedn. Proc., 34, 945 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SILVER, J., HOOD, L. An approach to amino acid sequence analysis of transplantation antigens. Nature 256, 63–64 (1975). https://doi.org/10.1038/256063a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/256063a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
