Abstract
THERE is now considerable interest in the construction of plasmids containing DNA fragments from a variety of bacterial genera, for purposes of studying the expression of the genetic potentialities of the incorporated bacterial DNA in different bacterial hosts. We report that the self-transmissible plasmid RP4 has only one site susceptible to cutting by the enzyme EcoRI, and should therefore be a very suitable bacterial DNA vector in such experiments.
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References
Hedgepeth, J., Goodman, H. M., and Boyer, H. W., Proc. natn. Acad. Sci. U.S.A., 69, 3448–3452 (1972).
Mertz, J. E., and Davis, R. W., Proc. natn. Acad. Sci. U.S.A., 69, 3370–3374 (1972).
Cohen, S. N., Chang, A. C. Y., Boyer, H. W., and Helling, R. B., Proc. natn. Acad. Sci. U.S.A., 70, 3240–3244 (1973).
Chang, A. C. Y., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 71, 1030–1034 (1974).
Tanaka, T., and Weisblum, B., J. Bact., 121, 354–362 (1975).
Report of the working party on the experimental manipulation of the genetic composition of micro-organisms (HMSO, Cmnd No. 5880, 1975).
Datta, N., Hedges, R. W., Shaw, E. J., Sykes, R. P., and Richmond, M. H., J. Bact., 108, 1244–1249 (1971).
Olsen, R. H., and Shipley, P., J. Bact., 113, 772–780 (1973).
Beringer, J., J. gen. Microbiol., 84, 188–198 (1974).
Hedges, R. W., Jacob, A. E., and Smith, J. T., J. gen. Microbiol., 84, 199–204 (1974).
Hedges, R. W., and Jacob, A. E., Molec. gen. Genet., 132, 31–40 (1974).
Dixon, R. A., Cannon, F. C., and Kondorosi, A., Proc. soc. Gen. Microbiol., 2, 43 (1975).
Sharp, P. A., Sugden, B., and Sambrook, J., Biochemistry, 12, 3055–3063 (1973).
Jacob, A. E., and Hobbs, S. J., J. Bact., 117, 360–372 (1974).
Morrow, J. F., et al., Proc. natn. Acad. Sci. U.S.A., 71, 1743–1747 (1974).
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JACOB, A., GRINTER, N. Plasmid RP4 as a vector replicon in genetic engineering. Nature 255, 504–506 (1975). https://doi.org/10.1038/255504a0
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DOI: https://doi.org/10.1038/255504a0
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