Abstract
ACTIN and myosin have been found in many non-muscle cells1. They are presumed to function in the generation of the force required in the various expressions of cytoplasmic or cell motility. Although both have been found in association with the plasma membrane2ā5, nothing is known about the nature or consequences of this association. Equally uncertain is the mechanism of movement of secretory granules : synaptic vesicles, for example, are transported along neurones at high rates (up to 20 cm dā1)6, probably in association with microtubules7. Roles for actomyosin in this process8 and in exocytosis9 have been proposed. We have used a new approach to the problem of actomyosin-membrane interaction, and report here the binding of actin and myosin in vitro to a purified cytoplasmic membrane component.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pollard, T. D., and Weihing, R. R., Critical Reviews in Biochemistry, 2, 1ā65 (CRC, Cleveland, Ohio, 1974).
Pollard, T. D., and Korn, E. D., J. biol. Chem., 248, 448ā450 (1973).
Spudich, J. A., J. biol. Chem., 249, 6013ā6020 (1974).
Kemp, R. B., Jones, B. M., and Grƶschel-Stewart, U., J. Cell Sci., 12, 631ā639 (1973).
Willingham, M. C., Ostlund, R. E., and Pastan, I., Proc. natn. Acad. Sci. U.S.A., 71, 4144ā4148 (1974).
Dahlstrƶm, A., Phil. Trans. R. Soc., B 261, 325ā358 (1971).
Heslop, J. P., Soc. exp. Biol. Symp., 28, 209ā227 (1974).
Ochs, S., Science, 176, 252ā260 (1970).
Berl, S., Puszkin, S., and Nicklas, W. J., Science, 179, 441ā446 (1973).
Geffen, L. B., Livett, B. G., and Rush, R. A., J. Physiol., Lond., 204, 593ā605 (1969).
Flatmark, T., Lagercrantz, H., Terland, O., Helle, K. B., and Stjƶrne, L., Biochim. biophys. Acta, 245, 249ā252 (1971).
Smith, A. D., and Winkler, H., Biochem. J., 103, 480ā482 (1967).
Poisner, A. M., Fedn Proc., 29, 545 (1970).
Kendrick-Jones, J., Phil. Trans. R. Soc., B 265, 183ā189 (1973).
Laemmli, U., Nature, 227, 680ā685 (1970).
Huxley, H. E., J. molec. Biol., 7, 281ā308 (1963).
Goll, D. E., Suzuki, A., Temple, A., and Holmes, G. R., J. molec. Biol., 67, 469ā488 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BURRIDGE, K., PHILLIPS, J. Association of actin and myosin with secretory granule membranes. Nature 254, 526ā529 (1975). https://doi.org/10.1038/254526a0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1038/254526a0
This article is cited by
-
Function of the cytoskeleton in cells with microridges from the oral epithelium of the carp Cyprinus carpio
Cell and Tissue Research (1994)
-
Actin cytoskeleton and calcium-ATPase in the process of abomasal mucus secretion in cattle
Cell and Tissue Research (1990)
-
Lateral diffusion of luminal membrane components during secretion in parotid acinar cells of the rat
Cell and Tissue Research (1990)
-
Identification of a secretory granule-binding protein as caldesmon
Nature (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
