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Immunoperoxidase staining of α-bungarotoxin binding sites in muscle endplates shows distribution of acetylcholine receptors

Naturevolume 254pages339341 (1975) | Download Citation

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Abstract

α-BUNGAROTOXiN (αBT) and similar toxins are specific ligands for the nicotinic acetylcholine (ACh) receptors in skeletal muscle1,2 fish electric organ3–5 and retina6 and other parts of the central nervous system7,8. Fluorescent9,10 and autoradiographic1,11,12 methods have been used to visualise the αBT binding sites in muscle and electric organ. We report, here an αBT-immunoperoxidase staining method for light and electron microscopy, which is relatively convenient and has high resolution. We have used this method to study the distribution of ACh receptors in skeletal muscle.

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Affiliations

  1. Laboratory of Biochemical Genetics, National Institutes of Health, Bethesda, Maryland, 20014

    • MATHEW P. DANIELS
    •  & ZVI VOGEL

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https://doi.org/10.1038/254339a0

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