Letter | Published:

Formation of triple-stranded bovine DNA in vitro

Naturevolume 254pages8687 (1975) | Download Citation

Subjects

Abstract

EVIDENCE for a triple-stranded complex of polynucleotides was reported for homopolymer RNAs by Felsenfeld et al.1 in 1957. They detected a drop in absorbancy at 260 nm for a mixture consisting of two parts polyadenylic acid (poly(rA)) and one part polyuridylic acid (poly(rU)), during a continuous variation mixing study of the two homopolymers. Addition of 0.1 M MgCl2 produced the greatest triple-strand formation with a minimum absorbance at 67 mol % poly(rU), 33 mol % poly(rA)2. Since this early work, many triple-stranded complexes have been identified, including combinations of homopolymers of deoxyribonucleotides and ribonucleotides; Riley et al.3 reported a triple strand formed by the homopolymers dA: dT: dT. They also reported the caesium sulphate buoyant densities of various double-stranded and triple-stranded homopolymers. In all cases, the triple-stranded homopolymer had a greater buoyant density than the analogous double-stranded homopolymer. We now report evidence for the formation of triple-stranded bovine DNA in vitro.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Felsenfeld, G., Davies, D. R., and Rich, A., J. Am. chem. Soc., 79, 2033 (1957).

  2. 2

    Felsenfeld, G., and Rich, A., Biochim. biophys. Acta, 26, 457 (1957).

  3. 3

    Riley, M., Maling, B., and Chamberlin, M., J. molec. Biol., 20, 359 (1966).

  4. 4

    Kay, E. R. M., Simmons, M. S., and Dounce, A. L., J. Am. chem. Soc., 74, 1724 (1952).

  5. 5

    Perlgut, L. E., and Hernandez, V., Biochim. biophys. Acta, 289, 169 (1972).

  6. 6

    Rich, A., Proc. natn. Acad. Sci. U.S.A., 46, 1044 (1960).

  7. 7

    Miles, H. T., and Frazier, J., Biochem. biophys. Res. Commun., 14, 21 (1961).

  8. 8

    Morgan, A. R., and Wells, R. D., J. molec. Biol., 37, 63 (1968).

  9. 9

    Zubay, G., Proc. natn. Acad. Sci. U.S.A., 48, 456 (1962).

  10. 10

    Jehle, H., and Parke, W. C., in Structural chemistry and molecular biology (edit. by Rich, A., and Davidson, N.), 400–408 (Freeman, San Francisco, 1968).

  11. 11

    Jehle, H., Proc. natn. Acad. Sci. U.S.A., 53, 1451 (1965).

Download references

Author information

Affiliations

  1. Department of Chemistry, California State University, Long Beach, California, 90840

    • L. E. PERLGUT
    • , D. L. BYERS
    • , R. S. JOPE
    •  & V. KHAMVINWATHNA

Authors

  1. Search for L. E. PERLGUT in:

  2. Search for D. L. BYERS in:

  3. Search for R. S. JOPE in:

  4. Search for V. KHAMVINWATHNA in:

About this article

Publication history

Received

Revised

Issue Date

DOI

https://doi.org/10.1038/254086a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.