Abstract
CULTURE of dissociated lymphoid cells in vitro enables the description of various characteristics of an immune response as manifested under defined conditions—but not the time course of evolution. Normal lymphoid cell cultures are usually short term, those of spleen or lymph node cells from common laboratory animals rarely surviving longer than 12–14 d. Lymphoblastoid or plasmocytoma lines, established in continuous cultures originated from cancerous or Epstein–Barr virus-bearing tissues, seem to have lost their capacity for responding to specific antigens. We have shown1,2 that calf lymphoid cells can be maintained in more prolonged cultures (up to three months) without losing their immunocompetence. We report results obtained using dissociated calf lymph node cell cultures surviving for more than 2 yr.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Panijel, J., Delpierre, M. A., and Delaunay, M. C., C.r. hebd. Séanc. Acad. Sci., Paris 272, 2986–2989 (1971).
Panijel, J., Jonard, J., Delpierre, M. A., and Delaunay, M. C., in Phylogenic and ontogenic study of the immune response and its contribution to the immunological theory (edit. by Panijel, J., and Liacopoulos, P.), 201–219 (INSERM, Paris, 1973).
Jonard, J., and Panijel, J., Europ. J. Immun., 3, 245–249 (1973).
Nossal, G. J. V., Bussard, A. E., Lewis, H., and Mazie, J. C., J. exp. Med., 131, 894–916 (1970).
Price, G. B., and Makinodan, J., J. Immun., 108, 403–412 (1972).
Smith, J. M., Proc. R. Soc., B 157, 115–127 (1963).
Orgel, L. E., Nature, 243, 441–445 (1973).
Sheldrake, A. R., Nature, 250, 381–385 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DELPIERRE, MA., PANIJEL, J. In vitro ageing and evolution of immunocompetence in long term normal lymphoid cell cultures. Nature 254, 62–63 (1975). https://doi.org/10.1038/254062a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/254062a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
