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Chromosomal variation and the establishment of somatic cell lines in vitro


WHEN somatic cells from a number of species are explanted in vitro, they may either completely fail to grow, or they may continue to divide for a limited number of generations, at a progressively lower rate and with decreasing plating efficiency. They will eventually die unless, from this “crisis”, a new population emerges with altered properties (see for example ref. 1). This new population, the established line, is usually capable of existing indefinitely and its growth rate and plating efficiency progressively increase until they stabilise at a level characteristic of each individual line.

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TERZI, M., HAWKINS, T. Chromosomal variation and the establishment of somatic cell lines in vitro. Nature 253, 361–362 (1975).

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