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Chromosomal variation and the establishment of somatic cell lines in vitro

Abstract

WHEN somatic cells from a number of species are explanted in vitro, they may either completely fail to grow, or they may continue to divide for a limited number of generations, at a progressively lower rate and with decreasing plating efficiency. They will eventually die unless, from this “crisis”, a new population emerges with altered properties (see for example ref. 1). This new population, the established line, is usually capable of existing indefinitely and its growth rate and plating efficiency progressively increase until they stabilise at a level characteristic of each individual line.

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References

  1. Todaro, G. J., and Green, H., J. Cell Biol., 17, 299–313 (1963).

    Article  CAS  Google Scholar 

  2. Martin, G. M., and Sprague, C. A., Science, 166, 761–763 (1969).

    Article  ADS  CAS  Google Scholar 

  3. Goldstein, S., and Lin, C. C., Expl Cell Res., 70, 436–439 (1972).

    Article  CAS  Google Scholar 

  4. Weiss, M. C., and Green, H., Proc. natn. Acad. Sci. U.S.A., 58, 1104–1109 (1967).

    Article  ADS  CAS  Google Scholar 

  5. Terzi, M., J. cell Physiol., 80, 359–366 (1972).

    Article  CAS  Google Scholar 

  6. Pera, F., and Rainer, B., Chromosoma, 42, 71–86 (1973).

    Article  CAS  Google Scholar 

  7. Defendi, V., and Stoker, M. G. P., Nature new Biol., 242, 24–26 (1973).

    Article  CAS  Google Scholar 

  8. Larizza, L., Simoni, G., Tredici, F., and De Carli, L., Mutat. Res., 25, 123–130 (1974).

    Article  CAS  Google Scholar 

  9. Diamond, L., Int. J. Cancer, 2, 143–152 (1967).

    Article  CAS  Google Scholar 

  10. Tsuda, T., Tohoku J. exp. Med., 86, 380–393 (1965).

    Article  CAS  Google Scholar 

  11. Popescu, N. C., and DiPaolo, J. A., Cytogenetics, 11, 500–507 (1972).

    Article  CAS  Google Scholar 

  12. Hughes, D. T., Nature, 217, 518–523 (1968).

    Article  ADS  CAS  Google Scholar 

  13. Allderdice, P. W., et al., J. Cell Sci., 12, 263–274 (1973).

    CAS  PubMed  Google Scholar 

  14. Tanamura, A., and Yerganian, G., Genetics, 41, 664–665 (1956).

    Google Scholar 

  15. Ohno, S., In vitro, 2, 46–57 (1966).

    Article  Google Scholar 

Download references

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TERZI, M., HAWKINS, T. Chromosomal variation and the establishment of somatic cell lines in vitro. Nature 253, 361–362 (1975). https://doi.org/10.1038/253361a0

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  • DOI: https://doi.org/10.1038/253361a0

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