SEVERAL procedures for enrichment of fungal mutants are known and have been reviewed1,2 but they have many limitations. Here we describe a new technique based on selective cell-wall lysis, which has wide applicability and is highly efficient for isolating mutants differing from the wild-type strain in growth requirements.
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FERENCZY, L., SIPICZKI, M. & SZEGEDI, M. Enrichment of fungal mutants by selective cell-wall lysis. Nature 253, 46–47 (1975). https://doi.org/10.1038/253046a0
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