Abstract
QUIESCENT, non-growing cultures of normal fibroblasts may arise as a result of absence of anchorage1,2 or deprivation either of serum macromolecules3–5 or of a variety of nutrients6. This state can also be induced by sulphated polysaccharides7,8 and we have reported the inhibition of growth of normal BHK/21 hamster fibroblasts in monolayer by dextran sulphate and heparin9 and the protection against this inhibition by purines10. Goto et al. have also found that dextran sulphate added to growing cultures markedly reduces the saturation density of established lines not transformed by viruses, but not that of primary embryonic fibroblasts11. The ability of many cells to grow in agar only after transformation with tumour viruses has been similarly ascribed to the inhibitory effects of sulphated polysaccharides8,12 and a close quantitative relationship has been reported between tumorigenicity and plating efficiency in agar13.
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References
Montagnier, L., Path. Biol., Paris, 14, 244–251 (1966).
Stoker, M., O'Neill, C., Berryman, S., and Waxman, V., Int. J. Cancer, 3, 683–693 (1968).
Todaro, G. J., Lazar, G. K., and Green, H., J. cell. comp. Physiol., 66, 325–333 (1965).
Temin, H., Int. J. Cancer, 3, 771–787 (1968).
Bürk, R. R., in Growth regulating substances for Animal Cells (edit. by Defendi, V., and Stoker, M.), 39–50 (Wistar Institute Press, Philadelphia, 1967).
Pardee, A. B., Proc. natn. Acad. Sci. U.S.A., 71, 1286–1290 (1974).
Temin, H., J. natn. Cancer Inst., 37, 168–175 (1966).
Montagnier, L., C.r. hebd. Séanc Acad. Sci. Paris, Serie D., 267, 921–924 (1968).
Clarke, G. D., and Stoker, M. G. P., Ciba Foundation Symposium, Growth control in Cell Cultures (edit. by Wolstenholme, G. E. W., and Knight, J.), 17–32 (Churchill, London, 1971).
Clarke, G. D., and Smith, D., J. cell. Physiol., 81, 125–131 (1973).
Goto, M., Kataoka, Y., Kimura, T., Goto, K., and Sato, H., Expl Cell Res., 82, 367–374 (1973).
Montagnier, L., C. r. hebd. Séanc Acad. Sci. Paris, Serie D., 268, 2218–2221 (1969).
Kakunga, T., and Kamahora, J., Biken J., 11, 313–332 (1968).
Bremerskov, V., Nature new Biol., 246, 174 (1973).
Elbein, A. D., Adv. Enzymol., 40, 29–64 (1974).
Birch, J. R., and Pirt, S. J., J. Cell Sci., 7, 661–670 (1970).
Bush, H., Proc. R. Soc. Med., 66, 1242–1243 (1973).
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CLARKE, G., SHEARER, M. & RYAN, P. Association of polyanion resistance with tumorigenicity and other properties in BHK/21 cells. Nature 252, 501–503 (1974). https://doi.org/10.1038/252501a0
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DOI: https://doi.org/10.1038/252501a0
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