Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

In vitro phagocytosis of bacteria by insect blood cells

Abstract

PHAGOCYTOSIS is an important component of vertebrate cellular immunity and involves several stages which have been described in detail previously1,2. In contrast, little is known about this process in insects even though recent work3,4, on intracellular killing of bacteria indicates that phagocytosis probably plays an important role in the defence reactions of insects. In vertebrates, the development of in vitro techniques has greatly facilitated studies on phagocytosis, but in insects such techniques are not generally available for studying the blood cells (haemocytes) in strictly controlled conditions. Here we describe for the first time the stages in the phagocytosis of bacteria by insect haemocytes, using a newly developed culture technique.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Essner, E., J. biophys. biochem. Cytol., 7, 329–343 (1960).

    Article  CAS  Google Scholar 

  2. Hirsch, J. G., in Microbial Pathogenicity in Man and Animals (edit. by Smith, H., and Pearce, J. H.) 59–74 (Cambridge University Press, 1972).

    Google Scholar 

  3. Anderson, R. S., Holmes, B., and Good, R. A., J. Invert. Path., 22, 127–135 (1973).

    Article  CAS  Google Scholar 

  4. Anderson, R. S., Holmes, B., and Good, R. A., Comp. Biochem. Physiol., 46 B, 595–602 (1973).

    CAS  Google Scholar 

  5. Price, C. D., and Ratcliffe, N. A., Z. Zellforsch., 147, 537–549 (1974).

    Article  CAS  Google Scholar 

  6. Hirsch, J. G., and Fedorko, M. E., J. Cell Biol., 38, 615–627 (1968).

    Article  CAS  Google Scholar 

  7. Cameron, G. R., J. Path. Bact., 38, 441–466 (1934).

    Article  Google Scholar 

  8. Devauchelle, G., Meynadier, G., and Vago, C., J. Ultrastruct. Res., 38, 134–148 (1972).

    Article  CAS  Google Scholar 

  9. Salt, G., in The Cellular Defense Reactions of Insects, Cambridge Monographs in Experimental Biology, 16, 9 (Cambridge University Press, 1970).

    Book  Google Scholar 

  10. Leutenegger, R., Virology, 32, 109–116 (1967).

    Article  CAS  Google Scholar 

  11. Grimstone, A. V., Rotheram, S., and Salt, G., J. Cell Sci., 2, 281–292 (1967).

    CAS  PubMed  Google Scholar 

  12. Zachary, D., and Hoffmann, J. A., Z. Zellforsch., 141, 55–73 (1973).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

RATCLIFFE, N., ROWLEY, A. In vitro phagocytosis of bacteria by insect blood cells. Nature 252, 391–392 (1974). https://doi.org/10.1038/252391a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/252391a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing