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Differential staining of human and mouse chromosomes in interspecific cell hybrids


VIRUS-MEDIATED fusion of somatic cells to produce interspecific cell hybrids has proved to be a most valuable technique for biological research1. Such crosses often undergo an initial fairly random loss of many chromosomes of one parent before karyotypic stability is reached. This property can be used to generate clones of cells each containing a small selection of human chromosomes in a background genotype of some other species. Comparisons of such cell lines provide a form of segregation analysis which can substitute for more traditional genetic methods, particularly in studies on man where classical genetic methodology is difficult to apply.

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BOBROW, M., CROSS, J. Differential staining of human and mouse chromosomes in interspecific cell hybrids. Nature 251, 77–79 (1974).

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