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Affinity labelling the acceptor site of the peptidyl transferase centre of the Escherichia coli ribosome

Abstract

WE have identified two 50S proteins, L2 and L26-L27, in the peptidyl (P) site of the peptidyl transferase centre of E. coli ribosomes1 BrAc-3H-Phe-tRNAphe, a peptidyl-tRNA affinity analogue, was shown to bind specifically to the P site and covalently react with only L2 and L26-L27. The ability of the same molecules of ribosome-bound BrAcPhe-tRNAphe to participate in dipeptide formation and covalent attachment to ribosomal proteins was the most compelling evidence for functional P site binding.

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EILAT, D., PELLEGRINI, M., OEN, H. et al. Affinity labelling the acceptor site of the peptidyl transferase centre of the Escherichia coli ribosome. Nature 250, 514–516 (1974). https://doi.org/10.1038/250514a0

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