Abstract
CELLS die if cooled either too rapidly or too slowly during freezing in the presence of dimethylsulphoxide (DMSO)1–4, but survive at an intermediate optimal cooling rate5. We have now obtained improved recovery of lymphocytes, lymphoid cell line cells and Chinese hamster tissue culture cells by interrupting rapid cooling with a timed exposure to a single subzero temperature. This method is simple and here we put forward some theoretical reasons for the improved cell recovery.
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FARRANT, J., KNIGHT, S., MCGANN, L. et al. Optimal recovery of lymphocytes and tissue culture cells following rapid cooling. Nature 249, 452–453 (1974). https://doi.org/10.1038/249452a0
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DOI: https://doi.org/10.1038/249452a0
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