Letter | Published:

Cumulative Cytostatic Effect of ICRF 159

Naturevolume 247pages487490 (1974) | Download Citation

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Abstract

THE antimitotic action of (±)-1,2-bis (3,5-dioxopiperazin-1-yl) propane (ICRF 159) has been extensively studied in vivo in human1 and animal tumours2,3, but its mode of action at the cellular and biochemical level has not yet been elucidated. Although it reduces the rates of DNA, RNA and protein synthesis of cells in vitro4, it has also been shown to block the cell generation cycle in the post-DNA synthesis (G2) phase5,6. Tumours whose growth is halted contain many large multinucleate cells not seen in control tumours. An attempt has therefore been made to see if the morphological changes produced by the drug in cells grown in vitro could provide an indication of its intracellular site of action and point towards possible mechanisms of action. The cells used were non-synchronised cultures of non-transformed hamster fibroblasts (Nil 8 cells), HeLa cells and fibroblasts derived from a transplantable fibrosarcoma in Syrian hamsters (T17 cells). They were exposed to single daily doses of ICRF 159 for 3 d.

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References

  1. 1

    Hellmann, K., Newton, K. A., Whitmore, D. N., Hanham, I. W. F., and Bond, J., Br. med. J., 1, 822 (1969).

  2. 2

    Creighton, A. M., Hellmann, K., and Whitecross, S., Nature, 222, 384 (1969).

  3. 3

    Hellmann, K., and Burrage, K., Nature, 224, 273 (1969).

  4. 4

    Creighton, A. M., and Birnie, G. D., Int. J. Cancer, 5, 47 (1970).

  5. 5

    Sharpe, H. B. A., Field, E. O., and Hellmann, K., Nature, 226, 524 (1970).

  6. 6

    Hellmann, K., and Field, E. O., J. natn. Cancer Inst., 44, 539 (1970).

  7. 7

    Robbins, E., Jentzsh, G., and Micali, A., J. Cell Biol., 36, 329 (1968).

  8. 8

    Ker, J. F. R., and Wyllie, A. H., Br. J. Cancer, 26, 239 (1972).

  9. 9

    Creaven, P. J., and Taylor, S. G., Proc. Am. Ass. Cancer Res., 14, 20 (1973).

  10. 10

    Blackett, N. M., and Adams, K., Br. J. Haemat., 23, 751 (1972).

  11. 11

    Bassleer, R., Acta chir. belg., 1, 18 (1971).

  12. 12

    Levis, A. G., Danielei, G. A., and Piccinni, E., Nature, 207, 608 (1965).

  13. 13

    Wheeler, G. P., Bowden, B. J., Adamson, D. J., and Vail, M. H., Cancer Res., 30, 100 (1970).

  14. 14

    Palyi, I., Gyesko, A., and Sugar, J., Cancer Chemother. Rep., 53, 115 (1969).

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Affiliations

  1. Department of Pathology, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX

    • R. C. HALLOWES
    •  & D. G. WEST
  2. Department of Cancer Chemotherapy, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX

    • K. HELLMANN

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https://doi.org/10.1038/247487a0

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