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Biological Sciences: Effects of Cycloheximide on RNA Polymerase Specific Activity and Encystment in Acanthamoeba castellanii

Nature volume 244, pages 220–222 (1973)Cite this article

Abstract

TROPHOZOITES of the protozoan Acanthamoeba castellanii (Neff) possess at least two distinct RNA polymerase activities which can be separated by DEAE-‘Sephadex’ A-50 chromatography1. These enzymes show optimum activities at different magnesium, manganese, and salt concentrations. Polymerase fraction I is also inhibited by cycloheximide, while polymerase fraction II is affected by rifampin and α-amanitin.

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References

  1. Rudick, V. L., and Weisman, R. A., Biochim. Biophys. Acta, 299, 91 (1973).

    Article  CAS  Google Scholar 

  2. Potter, J. L., and Weisman, R. A., Develop. Biol., 28, 472 (1972).

    Article  CAS  Google Scholar 

  3. Neff, R. J., Ray, S. A., Benton, W. F., and Wilborn, M., Methods in Cell Physiology, 1 (edit. by Prescott, D. M.), 55 (Academic Press, 1964).

    Google Scholar 

  4. Horgen, P. A., and Griffin, D. H., Proc. US Nat. Acad. Sci., 68, 338 (1971).

    Article  ADS  CAS  Google Scholar 

  5. Horgen, P. A., J. Bact., 106, 281 (1971).

    Article  CAS  Google Scholar 

  6. Willems, M., Penman, M., and Penman, S., J. Cell Biol., 41, 177 (1969).

    Article  CAS  Google Scholar 

  7. Muramatsu, M., Shimada, N., and Higashinakagawa, T., J. Mol. Biol., 53, 91 (1970).

    Article  CAS  Google Scholar 

  8. Wanka, F., and Schrauwen, P. J. A., Biochim. Biophys. Acta, 254, 237 (1971).

    Article  CAS  Google Scholar 

  9. Higashinakagawa, T., and Muramatsu, M., Biochem. Biophys. Res. Commun., 47, 1 (1972).

    Article  CAS  Google Scholar 

  10. Roeder, R. G., and Rutter, W. J., Proc. US Nat. Acad. Sci., 65, 675 (1970).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, The University of Texas Health Science Center, San Antonio, Texas, 78284

    V. L. RUDICK & R. A. WEISMAN

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  1. V. L. RUDICK
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  2. R. A. WEISMAN
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RUDICK, V., WEISMAN, R. Biological Sciences: Effects of Cycloheximide on RNA Polymerase Specific Activity and Encystment in Acanthamoeba castellanii. Nature 244, 220–222 (1973). https://doi.org/10.1038/244220a0

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