Abstract
THE snake venom protein α-bungarotoxin binds with specificity and high affinity to nicotinic acetylcholine receptors of striated muscle1–6, electric tissues7–9, and mammalian brain10. For these reasons, this toxin, as well as similar toxins from elapid snakes, has been used for labelling, purifying and assessing the physicochemical properties of cholinergic receptors.
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References
Chang, C. C., and Lee, C. Y., Arch. Intern. Pharmacodyn., 144, 241 (1963).
Lee, C. Y., and Chang, C. C., Mem. Inst. Butantan Simp. Internac., 33, 555 (1966).
Miledi, R., and Potter, L. T., Nature, 233, 599 (1971).
Barnard, E. A., Wieckowski, J., and Chiu, T. H., Nature, 234, 207 (1971).
Fambrough, D. M., and Hartzell, H. C., Science, 176, 189 (1972).
Berg, D. K., Kelly, R. B., Sargent, P. B., Williamson, P., and Hall, Z. W., Proc. US Nat. Acad. Sci., 69, 147 (1972).
Changeux, J.-P., Kasai, M., and Lee, C. Y., Proc. US Nat. Acad. Sci., 67, 1241 (1970).
Miledi, R., Molinoff, P., and Potter, L. T., Nature, 229, 554 (1971).
Fiszer, De Plazas, S., and De Robertis, E., Biochim. Biophys. Acta, 274, 258 (1972).
Bosmann, H. B., J. Biol. Chem., 247, 130 (1972).
Varon, S., in The Neurosciences Second Study Program (edit. by Schmitt, F. O.), 83 (Rockefeller University Press, New York 1970).
Herschman, H., in Research Methods in Neurochemistry (edit. by Rodnight, R., and Marks, N.), 2 (Plenum, in the press).
Volle, R. L., and Koelle, G. B., in The Pharmacological Basis of Therapeutics, fourth ed. (edit. by Goodman, L. S., and Gilman, A.), 585 (Macmillan, New York, 1970).
Phillis, J. W., The Pharmacology of Synapses, 123 (Pergamon, Oxford, 1970).
Varon, S., and Raiborn, C., J. Neurocytol. (in the press).
Levi-Montalcini, R., and Angeletti, P. U., Develop. Biol., 7, 653 (1963).
Varon, S., Nomura, J., and Shooter, E. M., Biochemistry, 6, 2202 (1967).
Dulbecco, R., and Vogt, M., J. Exp. Med., 99, 167 (1954).
Vogel, Z., Sytkowski, A. J., and Nirenberg, M. W., Proc. US Nat. Acad. Sci., 69, 3180 (1972).
Sytkowski, A. J., Vogel, Z., and Nirenberg, M. W., Proc. US Nat. Acad. Sci., 70, 270 (1973).
Bourgeois, J.-P., Ryter, A., Menez, A., Fromageot, P., Boquet, P., and Changeux, J.-P., FEBS Lett., 25, 127 (1972).
Paton, W. D. M., and Zaimis, E. J., Brit. J. Pharmacol., 4, 381 (1949).
Burn, J. H., and Rand, M. J., Ann. Rev. Pharmacol., 5, 163 (1965).
Nachmansohn, D., Proc. US Nat. Acad. Sci., 68, 3170 (1971).
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GREENE, L., SYTKOWSKI, A., VOGEL, Z. et al. α-Bungarotoxin used as a Probe for Acetylcholine Receptors of Cultured Neurones. Nature 243, 163–166 (1973). https://doi.org/10.1038/243163a0
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DOI: https://doi.org/10.1038/243163a0
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