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The Use of Phospholipid Film for Shaping Cell Cultures

Nature volume 242, pages 200–201 (1973)Cite this article

Abstract

IT would be useful for many studies of cell–cell interaction (for example, contact inhibition of movement, topoinhibition, electrical coupling) to be able to prepare cell cultures of various defined shapes and sizes. This can be done by protecting the whole surface of the culture substrate, except for desired regions, with a non-adhesive substance. The cells would then adhere and proliferate only in these regions. The protective substance must be (i) non-adhesive for cells; (ii) non-toxic for cells; (iii) insoluble in the media and (iv) strongly attached to the underlying substrate so that neither the size nor shape of adhesive region would change during cultivation. Lieberman et al.1 used dried agar film on a collagen layer as the non-adhesive substance for cardiac muscle cells.

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Authors and Affiliations

  1. Corpus “A”, Lomonosov State University, Moscow, 117234

    O. YU. IVANOVA & L. B. MARGOLIS

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  1. O. YU. IVANOVA
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  2. L. B. MARGOLIS
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IVANOVA, O., MARGOLIS, L. The Use of Phospholipid Film for Shaping Cell Cultures. Nature 242, 200–201 (1973). https://doi.org/10.1038/242200a0

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