Abstract
SYNAPTOSOMES prepared from the cerebral cortex by homogenization and density gradient centrifugation show a variety of metabolic properties characteristic of whole cells when incubated in suitable media1,2. Increasing the potassium concentration of the medium or electrical stimulation caused a preferential calcium dependent3 release of the physiologically active amino-acids glutamate, aspartate and GABA4. Acetylcholine synthesized by synaptosomes was also released by K+ and electrical stimulation5,6, and was also calcium dependent6. It has been shown7 that nerve-endings isolated from the median eminence of the hypothalamus contain corticotrophin-releasing factor (CRF) and antidiuretic hormone (ADH), and this study was undertaken to establish whether electrical and potassium stimulation would cause the release of neurosecretory substances and amino-acids from hypothalamic synaptosomes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bradford, H. F., J. Neurochem., 16, 675 (1969).
Bradford, H. F., and Thomas, A., J. Neurochem., 16, 1495 (1969).
de Belleroche, J. S., and Bradford, H. F., J. Neurochem., 19, 585 (1972).
Bradford, H. F., Brain Res., 19, 239 (1970).
Haga, T., J. Neurochem., 18, 781 (1971).
de Belleroche, J. S., and Bradford, H. F., J. Neurochem., 19, 1817 (1972).
Mulder, A. H., Geuze, J. J., and de Wied, D., Endocrinology, 87, 61 (1970).
Gray, E. G., and Whittaker, V. P., J. Anat., 96, 79 (1962).
Cheifetz, P. N., Acta Endocrinologica (in the press).
Dekanski, J., Br. J. Pharmac. Chemother., 7, 567 (1952).
Guillemin, R., Clayton, G. W., Smith, J. S., and Lipscomb, H. S., Endocrinology, 63, 348 (1958).
Dodd, P. R., Bradford, H. F., and Chain, E. B., Biochem. J., 125, 1027 (1971).
Morgan, I. G., and Austin, L., J. Neurochem., 15, 41 (1968).
Abdel-Latif, A. A., Yamaguchi, T., Yamaguchi, M., and Chang, F., Brain Res., 10, 307 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
EDWARDSON, J., BENNETT, G. & BRADFORD, H. Biological Sciences: Release of Amino-acids and Neurosecretory Substances after Stimulation of Nerve-endings (Synaptosomes) isolated from the Hypothalamus. Nature 240, 554–556 (1972). https://doi.org/10.1038/240554a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/240554a0
This article is cited by
-
Properties of LHRH release from a hypothalamic synaptosomal fraction of estrogen-primed ovariectomized rats
Neurochemical Research (1980)
-
Modulation of corticotrophin-releasing factor release from hypothalamic synaptosomes
Nature (1974)
-
Catecholamines in the Median Eminence: New Evidence for a Major Noradrenergic Input
Nature (1973)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
