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Lymphomas

Junctional adhesion molecule C (JAM-C) distinguishes CD27+ germinal center B lymphocytes from non-germinal center cells and constitutes a new diagnostic tool for B-cell malignancies

Leukemia volume 21, pages 1285–1293 (2007)Cite this article

Abstract

Differentiation of naïve B cells into plasma cells or memory cells occurs in the germinal centers (GCs) of lymph follicles or alternatively via a GC- and T-cell-independent pathway. It is currently assumed that B-cell lymphomas correlate to normal B-cell differentiation stages, but the precise correlation of several B-cell lymphomas to these two pathways remains controversial. In the present report, we describe the junctional adhesion molecule C (JAM-C), currently identified at the cell–cell border of endothelial cells, as a new B-cell marker with a tightly regulated expression during B-cell differentiation. Expression of JAM-C in tonsils allows distinction between two CD27+ B-cell subpopulations: JAM-C− GC B cells and JAM-C+ non-germinal B cells. The expression of JAM-C in different B-cell lymphomas reveals a disease-specific pattern and allows a clear distinction between JAM-C− lymphoproliferative syndromes (chronic lymphocytic leukemia, mantle cell lymphoma and follicular lymphoma) and JAM-C+ ones (hairy cell leukemia, marginal zone B-cell lymphoma). Therefore, we propose JAM-C as a new identification tool in B-cell lymphoma diagnosis.

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Acknowledgements

We are thankful to D Wohlwend (FACS facility, CMU, Geneva), M Donquier (Genomics platform, CMU, Geneva) and P Ropraz for their technical assistance; B Huard for supplying reagents; and P Bradfield for critical reading of this paper. This study was financially supported to TM by Swiss Cancer League No. OCS-01343-02-2003 and OCS-01781-08-2005; Foundation Dr Henri Dubois-Ferrière et Dinu Lipatti; and to BI by Swiss National Science Foundation No 3100.108099; Swiss Cancer league No. OCS-01335-02-2003.

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Authors and Affiliations

  1. Department of Pathology and Immunology, University Medical Center, Geneva, Switzerland

    C Ody, M Aurrand-Lions & B A Imhof

  2. Division of Clinical Pathology and Division of Hematology, Laboratory of Flow Cytometry, University Hospital, Geneva, Switzerland

    S Jungblut-Ruault, D Cossali & T Matthes

  3. Division of Hematology, University Hospital, Geneva, Switzerland

    M Barnet

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  1. C Ody
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Correspondence to T Matthes.

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Ody, C., Jungblut-Ruault, S., Cossali, D. et al. Junctional adhesion molecule C (JAM-C) distinguishes CD27+ germinal center B lymphocytes from non-germinal center cells and constitutes a new diagnostic tool for B-cell malignancies. Leukemia 21, 1285–1293 (2007). https://doi.org/10.1038/sj.leu.2404689

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