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Bedside RNA stabilizing kit systems for gene expression analysis of acute leukemias: influence of non-neoplastic white blood cells

Leukemia volume 19, pages 136–137 (2005)Cite this article

A Corrigendum to this article was published on 24 March 2005

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TO THE EDITOR

Gene expression profiling by quantitative real-time reverse transcriptase-polymerase chain reaction (RQ-PCR) or microarray technologies has become an important tool of research within the last decade.1, 2, 3 Among other obstacles, researchers using these techniques have to deal with the extraordinary instability of RNA ex vivo, which may result in marked differences of gene expression profiles.4 In acute leukemias, peripheral blood is frequently used as a source of malignant cells. In daily practice, the percentage of blast cells is increased by the density gradient centrifugation (DGC) followed by immediate RNA stabilization with various RNA stabilizing agents, thereby ensuring stable gene expression results. However, the time passing by between the collection of blood specimens and DGC remains the critical step as RNA degrades gradually during that period.

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  1. Division of Hematology, Department of Medicine, Medical University of Graz, Graz, Austria

    A Zebisch, W Linkesch & H Sill

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  1. A Zebisch
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Correspondence to H Sill.

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Zebisch, A., Linkesch, W. & Sill, H. Bedside RNA stabilizing kit systems for gene expression analysis of acute leukemias: influence of non-neoplastic white blood cells. Leukemia 19, 136–137 (2005). https://doi.org/10.1038/sj.leu.2403581

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