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Improvement of gene expression analysis by RQ-PCR technology: addition of BSA

Leukemia volume 18, pages 1022–1025 (2004)Cite this article

TO THE EDITOR

Real-time quantitative PCR (RQ-PCR) is used for diagnosis and detection of minimal residual disease (MRD) in bone marrow (BM) and peripheral blood (PB) of patients with acute lymphoblastic leukemia (ALL). Quantitative PCR analysis can be performed using tumour-specific DNA targets (eg Immunoglobin gene Rearrangements or IgR) but also using tumor specific RNA targets (eg fusion gene transcripts or FG).1 Like IgR, the FG could be very informative in MRD follow-up, although IgR are the reflection of the cellular load whereas the FG are the reflection of the gene expression.

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Authors and Affiliations

  1. Department of Hematological Biology,Hôpital Nord, Marseille, France

    C Picard

  2. Department of Biochemistry and Molecular Biology, Hôpital Universitaire Nord, ERT-MEIDIA, IFR Jean Roche, Université de la Méditerranée, Marseille, France

    M Silvy, G Pic & J Gabert

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  1. M Silvy
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  2. G Pic
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  3. J Gabert
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  4. C Picard
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Correspondence to J Gabert.

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Silvy, M., Pic, G., Gabert, J. et al. Improvement of gene expression analysis by RQ-PCR technology: addition of BSA. Leukemia 18, 1022–1025 (2004). https://doi.org/10.1038/sj.leu.2403339

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