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Interactive ChimerTrack software facilitates computation, visual displays and long-term tracking of chimeric status based on STRs

Leukemia volume 18, pages 909–911 (2004)Cite this article

Chimerism testing has become an important service provided by the post-transplantation monitoring laboratory. This is because chimeric status serves as a useful indication of the cellular dynamics involved in engraftment, or relapse of a malignant recipient cell population, particularly following stem cell transplantation. Previous reports in this Debate Round-Table on chimerism testing1,2,3,4,5 reflect the current interest in quantitatively estimating chimeric status based on an analysis of microsatellite markers, or short tandem repeats (STRs). The principal advantage of this approach is its high sensitivity coupled with the potential quantitative character of the results. The latter affords a most useful capability to follow a patient's chimeric status over time, that is, long-term trends.

Despite sophisticated technological approaches for analyzing DNA, the final phase of the process – when % chimerism is calculated – usually relies on manual transcription of the sequencer results followed by manual computation. Moreover, although STR data are well suited for long-term profiling of chimeric status in a patient, a manual retrospective graphic analysis comparing the present to previous samples is not a feasible undertaking for every patient sample.

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Authors and Affiliations

  1. Immunogenetics and Histocompatibility/Tissue Typing Laboratory, Rabin Medical Center, Petach Tikvah, Israel

    D Kristt & T Klein

  2. Bone Marrow Transplantation Unit, Schneider Children's Medical Center, Petach Tikvah, Israel

    J Stein & I Yaniv

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  1. D Kristt
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  2. J Stein
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  3. I Yaniv
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  4. T Klein
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Correspondence to D Kristt.

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Kristt, D., Stein, J., Yaniv, I. et al. Interactive ChimerTrack software facilitates computation, visual displays and long-term tracking of chimeric status based on STRs. Leukemia 18, 909–911 (2004). https://doi.org/10.1038/sj.leu.2403325

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