Abstract
THE discovery that messenger RNA (mRNA) in mammalian cells contains sequences of polyadenylic acid (poly A) about 200 nucleotides long localized at the 3′-OH terminus of the mRNA molecule1–5 has raised questions about the function of poly A, but has also provided a powerful new tool for the study of mRNA metabolism. I have measured the stability of mRNA in exponentially growing mouse L-cells without having to resort to the use of inhibitors of RNA synthesis, and found that poly A-containing mRNA has a half-life of 10 h, and thus turns over approximately once per cell generation (15 h). This high stability is in striking contrast to the 3–4 h half-life for mRNA in cultured cells obtained by following the decay of polyribosomes after treatment with a high dose of actinomycin D6,7, This, however, agrees with the finding of Cheevers and Sheinin8 that there is a long-lived fraction of mRNA in cells treated with a low dose of actinomycin D. Singer and Penman9 have simultaneously found that poly A-containing mRNA in HeLa cells turns over about once per cell generation.
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References
Kates, J., Cold Spring Harbor Symp. Quant. Biol., 35, 743 (1970).
Lee, S. Y., Mendecki, J., and Brawerman, G., Proc. US Nat. Acad. Sci., 68, 1331 (1971).
Edmonds, M., Vaughan, jun., M. H., and Nakazoto, H., Proc. US Nat. Acad. Sci., 68, 1336 (1971).
Darnell, J. E., Wall, R., and Tushinski, R. J., Proc. US Nat. Acad. Sci., 68, 1321 (1971).
Perry, R. P., Greenberg, J. R., Kelley, D. E., LaTorre, J., and Schochetman, G., Gene Expression and Its Regulation (Plenum Press).
Penman, S., Scherrer, K., Becker, Y., and Darnell, J. E., Proc. US Nat. Acad. Sci., 49, 654 (1963).
Craig, N., Perry, R. P., and Kelley, D. E., Biochim. Biophys. Acta, 246, 493 (1971).
Cheevers, W. P., and Sheinin, R., Biochim. Biophys. Acta, 204, 449 (1970).
Singer, R. H., and Penman, S., Nature New Biology (in the press).
Rake, A. V., and Graham, A. F., J. Cell. Comp. Physiol., 59, 139 (1962).
Perry, R. P., and Kelley, D. E., J. Mol. Biol., 35, 37 (1968).
Weber, M. J., Nature New Biology, 235, 58 (1972).
Perry, R. P., LaTorre, J., Kelley, D. E., and Greenberg, J. R., Biochim. Biophys. Acta, 262, 220 (1972).
Adesnik, M., and Darnell, J. E., J. Mol. Biol. (in the press).
Borun, T. W., Scharff, M. D., and Robbins, E., Proc. US Nat. Acad. Sci., 58, 1977 (1967).
Gallwitz, D., and Mueller, G. C., J. Biol. Chem., 244, 5947 (1969).
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GREENBERG, J. High Stability of Messenger RNA in Growing Cultured Cells. Nature 240, 102–104 (1972). https://doi.org/10.1038/240102a0
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DOI: https://doi.org/10.1038/240102a0
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