Abstract
DURING the early implantation stage in the mouse several events in the differentiation of the embryo are initiated. Once the zona pellucida has been lost, attachment to the uterine epithelium occurs, the inner cell mass comes to assume its mesometrial orientation1, trophoblastic giant cells develop, differentiation of the endoderm begins and the inner cell mass undergoes changes leading to egg cylinder formation. The in vitro culture of the attachment stage would allow direct observation and analysis of some of these events and an experimental investigation of the conditions influencing them. Retransfer of eggs to a receptive host animal in order to follow through the effects of experimental manipulations on earlier stages could also, in some cases, be eliminated if in vitro development of early implantation stages could be obtained.
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References
Kirby, D. R. S., Potts, D. M., and Wilson, I. B., J. Embryol. Exp. Morphol., 17, 527 (1967).
Cole, R. J., and Paul, J., in Pre-Implantation Stages of Pregnancy (edit. by Wolstenholme, G. E. W., and O'Connor, M.), Ciba Foundation Symp. (Churchill, London, 1965).
Kirby, D. R. S., J. Reprod. Fertil., 5, 1 (1963).
Stevens, L. C. J., Embryol. Exp. Morphol., 20, 339 (1968).
Tarkowski, A. K., J. Embryol. Exp. Morphol., 10, 476 (1962).
Cole, R. J., J. Embryol. Exp. Morphol., 17, 481 (1967).
Potts, D. M., and Wilson, I. B., J. Anat., 102, 1 (1967).
Carter, S. B., Nature, 206, 1183 (1965).
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JENKINSON, E., WILSON, I. In Vitro Support System for the Study of Blastocyst Differentiation in the Mouse. Nature 228, 776–778 (1970). https://doi.org/10.1038/228776a0
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DOI: https://doi.org/10.1038/228776a0
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