Separation of Parasites in Sucrose Gradients


SUCROSE gradients have been used widely for the separation of cellular and subcellular particles, but rarely for the separation of whole organisms either from one another or from debris. Red blood cells, infected with malaria and trypanosomes, can be separated from uninfected cells in sucrose gradients after low speed centrifugation1. This simple technique can also be applied to various parasitic Protozoa and Metazoa.


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    Williamson, J., and Cover, B., Trans. Roy. Soc. Trop. Med. Hyg., 60, 425 (1966).

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COX, F. Separation of Parasites in Sucrose Gradients. Nature 227, 192 (1970).

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