Abstract
Ribosomes from E. coli initiate synthesis in vitro of all three of the proteins coded by phage f2, but ribosomes from B. stearothermophilus initiate synthesis of only one. The specificity of initiation depends only on the source of the 30S subunits; the origin of the 50S sub-units and initiation factors has no effect.
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References
Anderson, J., Dahlberg, J., Bretscher, M., Revel, M., and Clark, B., Nature, 216, 1072 (1967).
Nomura, M., and Lowry, C. J., Proc. US Nat. Acad. Sci., 58, 946 (1967).
Revel, M., Lelong, J. C., Brawerman, G., and Gros, F., Nature, 219, 1016 (1968).
Iwasaki, K., Sabol, S., Wahba, A. J., and Ochoa, S., Arch. Biochem. Biophys., 1255, 42 (1968).
Greenshpan, H., and Revel, M., Nature, 224, 331 (1969).
Clark, B., and Marcker, K., Nature, 211, 378 (1966).
Ghosh, H. P., Soll, D., and Khorana, H. G., J. Mol. Biol., 25, 275 (1967).
Lodish, H. F., Nature, 224, 867 (1969).
Lodish, H. F., and Robertson, H. D., Cold Spring Harbor Symp. Quant. Biol., 34 (in the press).
Lodish, H. F., and Robertson, H., J. Mol. Biol., 45, 9 (1969).
Capecchi, M., J. Mol. Biol., 21, 173 (1966).
Vinuela, E., Salas, M., and Ochoa, S., Proc. US Nat. Acad. Sci., 57, 729 (1967).
Steitz, J. A., Nature, 224, 957 (1970).
Brawerman, G., Revel, M., Salser, W., and Gros, F., Nature, 223, 957 (1969).
Revel, M., Greenshpan, H., and Herzberg, M. (in the press).
Lodish, H. F., Nature, 220, 345 (1968).
Lodish, H. F., Biochem. Biophys. Res. Commun., 37, 127 (1969).
Lodish, H. F., J. Mol. Biol. (in the press).
Kolakofsky, D., Nature, 220, 244 (1968).
Guthrie, C., and Nomura, M., Nature, 219, 232 (1968).
Hindley, J., and Staples, D. H., Nature, 224, 964 (1969).
Adams, J. M., Jeppesen, P. G. N., Sanger, F., and Barrell, B., Nature, 223 1009 (1969).
Nichols, J. N., Nature, 225, 147 (1970).
Revel, M., Herzberg, M., and Greenshpan, H., Cold Spring Harbor Symp. Quant. Biol., 34 (in the press).
Lodish, H. F., J. Mol. Biol., 32, 681 (1968).
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LODISH, H. Specificity in Bacterial Protein Synthesis : Role of Initiation Factors and Ribosomal Subunits. Nature 226, 705–707 (1970). https://doi.org/10.1038/226705a0
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DOI: https://doi.org/10.1038/226705a0
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