Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Genetic Analysis of an E. coli Strain with a Mutation affecting DNA Polymerase


The mutation affecting DNA polymerase activity in the mutant strain isolated by De Lucia and Cairns is located between metE and rha, at approximately 75 minutes on the Escherichia coli chromosome. The mutation is recessive to the wild-type gene in partial diploids. It is an amber nonsense mutation which responds to the suppressors Sul+, Sull+ and Sulll+. Strains carrying the mutation are not deficient in carrying out genetic recombination.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. De Lucia, P., and Cairns, J., Nature, 224, 1164 (1969).

    ADS  CAS  Article  Google Scholar 

  2. Willson, C., Perrin, D., Cohn, M., Jacob, F., and Monod, J., J. Mol. Biol., 8, 582 (1964).

    CAS  Article  Google Scholar 

  3. Scaife, J., and Gross, J. D., Genet. Res. Camb., 4, 328 (1963).

    Article  Google Scholar 

  4. Taylor, A. L., and Trotter, C. D., Bact. Rev., 31, 332 (1967).

    CAS  Google Scholar 

  5. Brenner, S., and Beckwith, J. R., J. Mol. Biol., 13, 629 (1965).

    Article  Google Scholar 

  6. Calender, R., and Lindahl, G., virology (in the press).

  7. Ogawa, H., Shimada, K., and Tomizawa, J., Mol. Gen. Genetics, 101, 227 (1968).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

GROSS, J., GROSS, M. Genetic Analysis of an E. coli Strain with a Mutation affecting DNA Polymerase. Nature 224, 1166–1168 (1969).

Download citation

  • Received:

  • Issue Date:

  • DOI:

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing