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Optical Activity of Biopolymers

Abstract

Theoretical and experimental advances have led to a reassessment of the interpretation of optical rotatory dispersion and circular dichroism of nucleic acids, proteins and related compounds. The present state of the field is reviewed in this article.

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References

  1. Moscowitz, A., Adv. Chem. Phys., 4, 67 (1962); Tinoco, I., Adv. Chem. Phys., 4, 113 (1962); Schellman, J. A., Acc. Chem. Res., 1, 144 (1968).

    Google Scholar 

  2. Carver, J. P., Schechter, E., and Blout, E. R., J. Amer. Chem. Soc., 88, 2550 (1966).

    Article  CAS  Google Scholar 

  3. Yang, J. T., Samejima, T., and Sarkar, P. K., Biopolymers, 4, 623 (1966).

    Article  CAS  Google Scholar 

  4. Samejima, T., and Yang, J. T., J. Biol. Chem., 240, 2094 (1965).

    PubMed  CAS  Google Scholar 

  5. Sarkar, P. K., and Yang, J. T., J. Biol. Chem., 240, 2088 (1965); Sarkar, P. K., and Yang, J. T., Biochemistry, 4, 1238 (1965).

    PubMed  CAS  Google Scholar 

  6. McMullen, D. W., Jaskunas, S. R., and Tinoco, I., Biopolymers, 5, 589 (1967); Samejima, T., Hashizume, H., Imahori, K., Fuji, K., and Miura, K-I., J. Mol. Biol., 34, 39 (1968).

    Article  CAS  PubMed  Google Scholar 

  7. Brahms, J., and Mommaerts, W. F. H. M., J. Mol. Biol., 10, 73 (1964).

    Article  CAS  PubMed  Google Scholar 

  8. Brahms, J., Maurizot, J-C., and Michelson, A. M., J. Mol. Biol., 25, 481 (1967).

    Article  CAS  PubMed  Google Scholar 

  9. Tinoco, I., J. Chim. Phys., 65, 91 (1968).

    Article  CAS  Google Scholar 

  10. Ts'o, P. O. P., Rapaport, S. A., and Bollum, F. J., Biochemistry, 5, 4153 (1966); Adler, A., Grossman, L., and Fasman, G. D., Proc. US Nat. Acad. Sci., 87, 423 (1967); Brahms, J., and Sadron, C., Nature, 212, 1309 (1966).

    Article  CAS  Google Scholar 

  11. Fasman, G. D., Lindblow, G., and Seaman, E., J. Mol. Biol., 12, 630 (1965); Gratzer, W. B., Biochim. Biophys. Acta, 123, 431 (1966).

    Article  CAS  PubMed  Google Scholar 

  12. Warshaw, M. M., and Tinoco, I., J. Mol. Biol., 20, 29 (1966); Warshaw, M. M., thesis Univ. California, Berkeley (1966).

    Article  CAS  PubMed  Google Scholar 

  13. Cantor, C. R., Jaskunas, S. R., and Tinoco, I., J. Mol. Biol., 20, 39 (1966); Cantor, C. R., and Tinoco, I., Biopolymers, 5, 84 (1967).

    Article  CAS  PubMed  Google Scholar 

  14. Cotter, R. I., McPhie, P., and Gratzer, W. B., Nature, 216, 864 (1967): Bush, C. A., and Scheraga, H. A., Biochemistry, 6, 3036 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Rich, A., and Tinoco, I., J. Amer. Chem. Soc., 82, 6409 (1960).

    Article  CAS  Google Scholar 

  16. Oriel, P. J., and Koening, J. A., Arch. Biochem., 127, 274 (1968).

    Article  CAS  PubMed  Google Scholar 

  17. Oriel, P. J., Arch. Biochem., 115, 577 (1966).

    Article  CAS  PubMed  Google Scholar 

  18. Cohen, P., and Kidson, C., J. Mol. Biol., 35, 241 (1968); Inoue, S., and Ando, T., Biochem. Biophys. Res. Commun., 32, 501 (1968).

    Article  CAS  PubMed  Google Scholar 

  19. Maestre, M. F., and Tinoco, I., J. Mol. Biol., 23, 323 (1967).

    Article  CAS  PubMed  Google Scholar 

  20. Tunis, M. J. B., and Hearst, J. E., Biopolymers, 6, 1218 (1968).

    Article  CAS  PubMed  Google Scholar 

  21. Arnott, S., Fuller, W., Hodgson, A., and Prutton, I., Nature, 220, 561 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Bode, V. C., and McHattie, L. A., J. Mol. Biol., 32, 673 (1968).

    Article  CAS  PubMed  Google Scholar 

  23. Lamborg, M. R., and Zamecnik, P. C., Biochem. Biophys. Res. Commun., 20, 328 (1965); Sarin, P. C., Zamecnik, P. C., Bergquist, P. L., and Scott, J. F., Proc. US Nat. Acad. Sci., 55, 579 (1966); Wolfe, F. H., Oikawa, K., and Kay, C. M., Biochemistry, 7, 3361 (1968).

    Article  CAS  PubMed  Google Scholar 

  24. Blake, A., and Peacocke, A. R., Biopolymers, 4, 1091 (1968), 5, 38 (1967); Yamaoka, K., and Resnick, R., J. Phys. Chem., 70, 4051 (1966), and Nature, 213, 1031 (1967); Gardner, B. J., and Mason, S. F., Biopolymers, 5, 79 (1967).

    Article  Google Scholar 

  25. Yamaoka, K., and Ziffer, H., Biochemistry, 7, 1001 (1968); Courtois, Y., Guschlbauer, W., and Fromageot, P., Europ. J. Biochem., 6, 106 (1968).

    Article  CAS  PubMed  Google Scholar 

  26. Moffitt, W., Proc. US Nat. Acad. Sci., 42, 736 (1956); Moffitt, W., and Yang, J. T., Proc. US Nat. Acad. Sci., 42, 596 (1956).

    Article  ADS  CAS  Google Scholar 

  27. Urnes, P., and Doty, P., Adv. Protein Chem., 16, 401 (1961).

    Article  CAS  PubMed  Google Scholar 

  28. Yamaoka, K., Biopolymers, 2, 219 (1964); Schechter, E., and Blout, E. R., Proc. US Nat. Acad. Sci., 51, 695 (1964); Carver, J. P., Schechter, E., and Blout, E. R., J. Amer. Chem. Soc., 88, 2562 (1966).

    Article  CAS  Google Scholar 

  29. Yang, J. T., Proc. US Nat. Acad. Sci., 53, 438 (1965); and in Poly-α-amino Acids (edit. by Fasman, G. D.), 239 (Dekker, New York, 1967).

    Google Scholar 

  30. Chignell, D. A., and Gratzer, W. B., Nature, 210, 262 (1966).

    Article  ADS  CAS  Google Scholar 

  31. Fasman, G. D., and Potter, J., Biochem. Biophys. Res. Commun., 27, 209 (1967).

    Article  CAS  PubMed  Google Scholar 

  32. Schellman, J. A., and Oriel, P. J., J. Chem. Phys., 37, 2114 (1962); Schellman, J. A., J. Chem. Phys., 44, 55 (1966).

    Article  ADS  CAS  Google Scholar 

  33. Litman, B. J., and Schellman, J. A., J. Chem. Phys., 69, 978 (1965); Balasubramanian, D., and Wetlaufer, D. B., J. Amer. Chem. Soc., 88, 3449 (1966); Urry, D. W., J. Phys. Chem., 72, 3035 (1968).

    Article  CAS  Google Scholar 

  34. Urry, D. W., Ann. Rev. Phys. Chem., 19, 477 (1968).

    Article  ADS  CAS  Google Scholar 

  35. Yang, J. T., and McCabe, W. J., Biopolymers, 3, 209 (1965).

    Article  CAS  PubMed  Google Scholar 

  36. Tanford, C., Kawahara, K., Lapanje, S., Hooker, T. M., Zarlengo, M. H., Salahuddin, A., Aune, K. C., and Takagi, T., J. Amer. Chem. Soc., 89, 5023 (1967).

    Article  CAS  Google Scholar 

  37. Rosenheck, K., and Sommer, B., J. Chem. Phys., 46, 532 (1967); Pysh, E., Proc. US Nat. Acad. Sci., 56, 825 (1966).

    Article  ADS  CAS  PubMed  Google Scholar 

  38. Pysh, E., J. Mol. Biol., 23, 587 (1967).

    Article  CAS  PubMed  Google Scholar 

  39. Bovey, F. A., and Hood, F. A., Biopolymers, 5, 325 (1967).

    Article  CAS  Google Scholar 

  40. Greenfield, N., Davidson, B., and Fasman, G. D., Biochemistry, 6, 1630 (1967).

    Article  CAS  PubMed  Google Scholar 

  41. Cathou, R. E., Hammes, G. G., and Schimmel, P. R., Biochemistry, 4, 2687 (1965); Greenfield, N. I., Davidson, B., and Fasman, G. D., Biochemistry, 6, 1630 (1967); Steiner, L. A., and Lowey, S., J. Biol. Chem., 241, 231 (1966); Dorrington, K. J., and Tanford, C., J. Biol. Chem., 243, 4745 (1968); Cathou, R. E., Kulczyck, A., and Haber, E., Biochemistry, 7, 3959 (1968); Timasheff, S. N., Townend, R., and Perlmann, G. E., J. Biol. Chem., 242, 2290 (1967); Jirgensons, B., J. Biol. Chem., 241, 147 (1966).

    Article  CAS  PubMed  Google Scholar 

  42. Craig, L. C., Proc. US Nat. Acad. Sci., 61, 152 (1968); Ichishima, E., and Yoshida, F., Biochim. Biophys. Acta, 147, 341 (1967); Franze de Fernandez, M. T., Delius, A. E., and Paladini, A. G., Biochim. Biophys. Acta, 154, 223 (1968).

    Article  ADS  CAS  Google Scholar 

  43. Schellman, J. A., and Lowe, M. J., J. Amer. Chem. Soc., 90, 1070 (1968).

    Article  CAS  Google Scholar 

  44. Beychok, S., Armstrong, J. McD., Lindblow, C., and Edsall, J. T., J. Biol. Chem., 241, 5150 (1966); Kronman, M. J., Blum, R., and Holmes, L. G., Biochem. Biophys. Res. Commun., 19, 227 (1965); Glazer, A. N., and Simmons, N. S., J. Amer. Chem. Soc., 87, 2087 (1965).

    PubMed  CAS  Google Scholar 

  45. Rosenberg, A., J. Biol. Chem., 241, 5119 (1966).

    PubMed  CAS  Google Scholar 

  46. Edelhoch, H., Lippoldt, R. E., and Wilchek, M., J. Biol. Chem., 243, 4799 (1968).

    PubMed  CAS  Google Scholar 

  47. Green, N. M., and Melamed, M., Biochem. J., 100, 614 (1966); Yang, C. C., Cheng, C. E., Hayashi, K., Suzuki, T., Ikeda, K., and Hamaguchi, K., Biochim. Biophys. Acta, 168, 373 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Velluz, L., and Legrand, M., Angew. Chem., 4, 838 (1965).

    Article  Google Scholar 

  49. Simpson, R. B., and Vallee, B. L., Biochemistry, 5, 2531 (1966); Simmons, N. S., and Glazer, A. N., J. Amer. Chem. Soc., 89, 5040 (1967).

    Article  CAS  PubMed  Google Scholar 

  50. Beychok, S., Proc. US Nat. Acad. Sci., 53, 999 (1965); Coleman, D. L., and Blout, E. R., J. Amer. Chem. Soc., 90, 2405 (1968).

    Article  ADS  CAS  Google Scholar 

  51. Urry, D. W., Quadrifoglio, F., Walter, R., and Schwartz, I. L., Proc. US Nat. Acad. Sci., 60, 967 (1968).

    Article  ADS  CAS  Google Scholar 

  52. Claeson, G., Acta Chem. Scand., 22, 2429 (1968).

    Article  CAS  Google Scholar 

  53. Tiffany, M. L., and Krimm, S., Biopolymers, 6, 1379 (1968).

    Article  CAS  PubMed  Google Scholar 

  54. Schuster, T. M., Biopolymers, 3, 681 (1965); Tomimatsu, Y., Vitello, L., and Gaffield, W., Biopolymers, 4, 653 (1966); Cassim, J. Y., and Yang, J. T., Biochem. Biophys. Res. Commun., 26, 58 (1967).

    Article  CAS  Google Scholar 

  55. Herskovits, T. T., Townend, R., and Timasheff, S. N., J. Amer. Chem. Soc., 86, 4445 (1964); Tanford, C., Buckley, C. E., De, P. K., and Lively, E. P., J. Biol. Chem., 237, 1168 (1962).

    Article  CAS  Google Scholar 

  56. Dratz, E. A., and Calvin, M., Nature, 211, 497 (1966); Ohta, T., Shimada, I., and Imahori, K., J. Mol. Biol., 26, 519 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  57. Scanu, A., and Hirz, R., Proc. US Nat. Acad. Sci., 59, 890 (1968); Gotto, A. B., Levy, R. I., and Frederiksen, D. S., Proc. US Nat. Acad. Sci., 60, 1436 (1968).

    Article  ADS  CAS  Google Scholar 

  58. Ulmer, D. D., and Vallee, B. L., Adv. Enzymol., 27, 37 (1965): Brady, A. H., and Beychok, S., Biochem. Biophys. Res. Commun., 32, 186 (1968); Takagi, T., Aki, K., Isemura, T., and Yamano, T., Biochem. Biophys. Res. Commun., 24, 501 (1966).

    PubMed  Google Scholar 

  59. Beychok, S., Tyuma, I., Benesch, R. E., and Benesch, R., J. Biol. Chem., 242, 2460 (1967); Javaherian, K., and Beychok, S., J. Mol. Biol., 37, 1 (1968).

    PubMed  CAS  Google Scholar 

  60. Urry, D. W., Proc. US Nat. Acad. Sci., 54, 640 (1965); Miles, D. W., and Urry, D. W., J. Biol. Chem., 243, 418 (1968).

    Article  ADS  CAS  Google Scholar 

  61. Pulido, P., Kägi, J. H. R., and Vallee, B. L., Biochemistry, 5, 1768 (1966); Tang, S. P. W., Coleman, J. E., and Myer, Y. P., J. Biol. Chem., 243, 4286 (1968).

    Article  CAS  PubMed  Google Scholar 

  62. Urry, D. W., and Ji, T. H., Arch. Biochem., 128, 802 (1968).

    Article  CAS  PubMed  Google Scholar 

  63. Stevens, L., Townend, R., Timasheff, S. N., Fasman, G. D., and Potter, J., Biochemistry, 7, 3717 (1968).

    Article  CAS  PubMed  Google Scholar 

  64. Kahn, P. C., and Beychok, S., J. Amer. Chem. Soc., 90, 4168 (1968).

    Article  CAS  Google Scholar 

  65. Sarkar, P. K., and Yang, J. T., in Conformation of Biopolymers (edit. by Ramachandran, G. N.), 1, 197 (Academic Press, 1967).

    Google Scholar 

  66. Brahms, J., Michelson, A. M., and Van Holde, K. E., J. Mol. Biol., 15, 467 (1966).

    Article  CAS  PubMed  Google Scholar 

  67. Brahms, J., Maurizot, J.-C., and Michelson, A. M., J. Mol. Biol., 28, 465 (1967).

    Article  Google Scholar 

  68. Holzwarth, G., and Doty, P., J. Amer. Chem. Soc., 87, 218 (1965); Quadrifoglio, F., and Urry, D. W., J. Amer. Chem. Soc., 90, 2755 (1968); Sarkar, P. K., and Doty, P., Proc. US Nat. Acad. Sci., 55, 981 (1966).

    Article  CAS  Google Scholar 

  69. Peggion, E., Cosani, A., Verdini, A. S., Del Pra, A., and Mammi, M., Biopolymers, 6, 1477 (1968).

    Article  CAS  PubMed  Google Scholar 

  70. Myer, Y. P., and McDonald, L. H., J. Amer. Chem. Soc., 89, 7142 (1967).

    Article  CAS  Google Scholar 

  71. Beychok, S., and Fasman, G. D., Biochemistry, 3, 1675 (1964).

    Article  CAS  PubMed  Google Scholar 

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GRATZER, W., COWBURN, D. Optical Activity of Biopolymers. Nature 222, 426–431 (1969). https://doi.org/10.1038/222426a0

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