Abstract
THE bulk of the incorporation of amino-acids into protein observable in isolated rat diaphragm takes place outside mitochondria1 as judged by the use of selective inhibitors of microsomal and mitochondrial incorporating activity2,3. The conclusions assume that negative results attainable with intact tissue do not merely indicate failure of adequate penetration. The latter is unlikely because (a) cycloheximide reduced incorporation virtually to zero, so confirming the microsomal origin of the incorporating activity, and (b) evidence to be presented here indirectly indicates an impressively rapid access to and penetration of mitochondria in cells by various agents of large molecular weight.
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MANCHESTER, K. Interference of Various Antibiotics and other Compounds with Protein Synthesis in Muscle and their Influence on Metabolic Regulation. Nature 221, 188–190 (1969). https://doi.org/10.1038/221188a0
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DOI: https://doi.org/10.1038/221188a0
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