Abstract
WHEN carrying out experiments with micro-organisms over a long period of time, say 5 years, it is extremely difficult to ensure that the source material remains unchanged. One method is to prepare a large number of ampoules of the freeze-dried micro-organisms and to use these for inoculation of cultures as required, but the large storage space necessary presents a difficulty, especially if more than one species of micro-organism is to be preserved. The number of ampoules necessary can be decreased if each ampoule is used to inoculate a cooked meat medium1, which is then cultured and stored at 4° C, but this method has limitations: viability decreases in cooked meat broth, and fresh broths have to be inoculated at intervals of about 3 months.
Similar content being viewed by others
Article PDF
References
The Oxoid Manual, third ed. (Oxoid Ltd, 1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cox, C. Method for the Routine Preservation of Micro-organisms. Nature 220, 1139 (1968). https://doi.org/10.1038/2201139a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2201139a0
This article is cited by
-
Experience with low temperature storage of bacteria and fungi
Infection (1974)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.