Abstract
A MICROELECTROPHORETIC technique for fractionation of RNA has already been described1. Further work showed that it was necessary to increase the resolution when microisolated RNA was subjected to electrophoresis. Although the fluid consistency of polyacrylamide gels of low percentage prevented their use on a microscale, it was found that polyacrylamide–agarose composite gels2 could be handled on a small scale and that sharp separation of RNA molecules is possible.
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References
Daneholt, B., Ringborg, U., Egyházi, E., and Lambert, B., Nature, 218, 292 (1968).
Peacock, A. C., and Dingman, C. W., Biochemistry, 7, 668 (1968).
Egyházi, E., and Hydén, H., Life Sci., 5, 1215 (1966).
Edström, J.-E., and Daneholt, B., J. Mol. Biol., 28, 331 (1967).
Edström, J.-E., in Methods in Cell Physiology (edit. by Prescott, D. M.), 417 (Academic Press, New York, 1964).
Loening, U. E., Biochem. J., 102, 251 (1967).
Wieme, R. J., Agar Gel Electrophoresis (Elsevier, Amsterdam, 1965).
Bishop, D. H. L., Claybrook, T. R., and Spiegelman, S., J. Mol. Biol., 26, 373 (1967).
Vincent, W. S., Baltus, E., and Lövlie, A., Nat. Cancer Inst. Monog., 23, 235 (1966).
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RINGBORG, U., EGYHÁZI, E., DANEHOLT, B. et al. Agarose-Acrylamide Composite Gels for Microfractionation of RNA. Nature 220, 1037–1039 (1968). https://doi.org/10.1038/2201037a0
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DOI: https://doi.org/10.1038/2201037a0
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