Abstract
BACTERIOPHAGES isolated from Brucella1–5 are all closely related3–6 and can be taken as replicas of the international reference type phage Tb of Popkhadze and Abashidze, which identifies the three main species of Brucella7: (1) Br. melitensis, which is unsusceptible (although variants sensitive to phage do occur); (2) Br. abortus, which is susceptible at “routine test dilution”, defined as the highest phage dilution producing a reaction of semi-confluent lysis on an appropriate indicator strain; and (3) Br. suis, which is susceptible only at concentrations ten thousand times greater than the routine test dilution. Highly concentrated suspensions of phage Tb produce ε spot of hazy opalescent lysis on lawns of Br. suis, which helps to distinguish it from the other two species.
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MOREIRA-JACOB, M. New Group of Virulent Bacteriophages showing Differential Affinity for Brucella Species. Nature 219, 752–753 (1968). https://doi.org/10.1038/219752a0
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DOI: https://doi.org/10.1038/219752a0
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