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Function of Three Protein Factors and Ribosomal Subunits in the Initiation of Protein Synthesis in E. coli

Nature volume 219, pages 10161021 (07 September 1968) | Download Citation

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Abstract

A study of the effects of the three initiation factors on the formation of the initiation complex has shown that factor C promotes AUG dependent binding of F-met-tRNAF to the free 30S ribosomal subunit, while factor A is required as well as factor C for the formation of the 70S-AUG-F-met-tRNAF complex. When natural mRNA is used as template, factor B has to be added to obtain binding of F-met-tRNAF in a configuration which allows peptide bond formation.

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References

  1. 1.

    , and , J. Mol. Biol., 8, 835 (1964).

  2. 2.

    , and , Proc. US Nat. Acad. Sci., 55, 147 (1966).

  3. 3.

    , and , J. Mol. Biol., 17, 394 (1966).

  4. 4.

    , and , Biochemistry, 5, 2777 (1966).

  5. 5.

    , , , and , Proc. US Nat. Acad. Sci., 56, 290 (1966).

  6. 6.

    , and , Biochem. Biophys. Res. Commun., 25, 124 (1966).

  7. 7.

    , and , J. Mol. Biol., 20, 123 (1966).

  8. 8.

    , , and , J. Mol. Biol., 31, 277 (1968).

  9. 9.

    , and , Proc. US Nat. Acad. Sci., 58, 1560 (1967).

  10. 10.

    , and , Biochem. Biophys. Res. Commun., 27, 12 (1967).

  11. 11.

    , , , and , J. Mol. Biol., 33, 231 (1968).

  12. 12.

    , Biochem. Biophys. Res. Commun., 26, 639 (1967).

  13. 13.

    , , and , Nature, 215, 588 (1967).

  14. 14.

    , and , Proc. US Nat. Acad. Sci., 57, 1050 (1967).

  15. 15.

    , and , Proc. US Nat. Acad. Sci., 58, 946 (1967).

  16. 16.

    , , , , and , Bull. Soc. Chim. Biol. (in the press).

  17. 17.

    , and , Nature, 211, 380 (1966).

  18. 18.

    , , , and , Arch. Biochem. Biophys. (in the press).

  19. 19.

    , and , Fed. Proc., 27, 398 (1968).

  20. 20.

    , and , Biochem. Biophys. Res. Commun., 30, 284 (1968).

  21. 21.

    , , , and , Nature, 217, 1013 (1968).

  22. 22.

    , , , and , Proc. US Nat. Acad. Sci., 58, 1652 (1967).

  23. 23.

    , J. Mol. Biol. (in the press).

  24. 24.

    , and , Science, 145, 1399 (1964).

  25. 25.

    , and , Biochem. Biophys. Res. Commun., 25, 233 (1966).

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Author notes

    • G. BRAWERMAN

    On leave of absence from Department of Biochemistry, Yale University School of Medicine.

Affiliations

  1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, Paris

    • M. REVEL
    • , J. C. LELONG
    • , G. BRAWERMAN
    •  & F. GROS

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https://doi.org/10.1038/2191016a0

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