Abstract
DESPITE the high intrinsic brilliance of some modern light sources, there are many occasions on which the intensity of an image viewed through a fluorescence microscope is inadequate, leading to inability to appreciate details or to inconveniently long exposures in photomicrography. This situation is likely to become increasingly important with the further development of more specific immunofluorescence methods and of fluorescence histochemistry. The system described here approaches the maximum theoretical efficiency and the image intensity is limited only by the intrinsic brilliance of the source and by the transmission or reflectivity of the optical components. At the same time this system opens up a number of new possibilities for viewing two different components simultaneously.
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BARER, R. Maximum-intensity Fluorescence Microscopy. Nature 217, 672 (1968). https://doi.org/10.1038/217672a0
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DOI: https://doi.org/10.1038/217672a0
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